Investigation of Clostridium botulinum group III's mobilome content

Cédric Woudstra; Caroline Le Maréchal; Rozenn Souillard; Fabrizio Anniballi; Bruna Auricchio; Luca Bano; Marie Hélène Bayon-Auboyer; Miriam Koene; Isabelle Mermoud; Roseane B. Brito; Francisco C.F. Lobato; Rodrigo O.S. Silva; Martin B. Dorner; Patrick Fach

doi:10.1016/j.anaerobe.2017.12.009

Investigation of Clostridium botulinum group III's mobilome content

Cédric Woudstra, Caroline Le Maréchal, Rozenn Souillard, Fabrizio Anniballi, Bruna Auricchio, Luca Bano, Marie Hélène Bayon-Auboyer, Miriam Koene, Isabelle Mermoud, Roseane B. Brito, Francisco C.F. Lobato, Rodrigo O.S. Silva, Martin B. Dorner, Patrick Fach^*

^*Corresponding author for this work

Epidemiology, Bioinformatics and Animal Studies

Research output: Contribution to journal › Article › Academic › peer-review

2 Citations (Scopus)

Abstract

Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete C. botulinum group III genome sequence (strain BKT015925), strains have been found to contain others mobile elements encoding for toxin components. In this study, seven assays targeting toxin genes present on the genetic mobile elements of C. botulinum group III were developed with the objective to better characterize C. botulinum group III strains. The investigation of 110 C. botulinum group III strains and 519 naturally contaminated samples collected during botulism outbreaks in Europe showed alpha-toxin and C2-I/C2-II markers to be systematically associated with type C/D bont-positive samples, which may indicate an important role of these elements in the pathogenicity mechanisms. On the contrary, bont type D/C strains and the related positive samples appeared to contain almost none of the markers tested. Interestingly, 31 bont-negative samples collected on farms after a botulism outbreak revealed to be positive for some of the genetic mobile elements tested. This suggests loss of the bont phage, either in farm environment after the outbreak or during laboratory handling.

Original language	English
Pages (from-to)	71-77
Journal	Anaerobe
Volume	49
DOIs	https://doi.org/10.1016/j.anaerobe.2017.12.009
Publication status	Published - 1 Feb 2018

Keywords

Animal botulism
Clostridium botulinum group III
Phage
Plasmid

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2 Citations
1 Article

Erratum to “Investigation of Clostridium botulinum group III's mobilome content”
Woudstra, C., Le Maréchal, C., Souillard, R., Anniballi, F., Auricchio, B., Bano, L., Bayon-Auboyer, M. H., Koene, M., Mermoud, I., Brito, R. B., Lobato, F. C. F., Silva, R. O. S., Dorner, M. B. & Fach, P., Jun 2019, In : Anaerobe. 57, p. 117-117
Research output: Contribution to journal › Article › Academic

Cite this

Woudstra, Cédric ; Le Maréchal, Caroline ; Souillard, Rozenn ; Anniballi, Fabrizio ; Auricchio, Bruna ; Bano, Luca ; Bayon-Auboyer, Marie Hélène ; Koene, Miriam ; Mermoud, Isabelle ; Brito, Roseane B. ; Lobato, Francisco C.F. ; Silva, Rodrigo O.S. ; Dorner, Martin B. ; Fach, Patrick. / Investigation of Clostridium botulinum group III's mobilome content. In: Anaerobe. 2018 ; Vol. 49. pp. 71-77.

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title = "Investigation of Clostridium botulinum group III's mobilome content",

abstract = "Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete C. botulinum group III genome sequence (strain BKT015925), strains have been found to contain others mobile elements encoding for toxin components. In this study, seven assays targeting toxin genes present on the genetic mobile elements of C. botulinum group III were developed with the objective to better characterize C. botulinum group III strains. The investigation of 110 C. botulinum group III strains and 519 naturally contaminated samples collected during botulism outbreaks in Europe showed alpha-toxin and C2-I/C2-II markers to be systematically associated with type C/D bont-positive samples, which may indicate an important role of these elements in the pathogenicity mechanisms. On the contrary, bont type D/C strains and the related positive samples appeared to contain almost none of the markers tested. Interestingly, 31 bont-negative samples collected on farms after a botulism outbreak revealed to be positive for some of the genetic mobile elements tested. This suggests loss of the bont phage, either in farm environment after the outbreak or during laboratory handling.",

keywords = "Animal botulism, Clostridium botulinum group III, Phage, Plasmid",

author = "C{\'e}dric Woudstra and {Le Mar{\'e}chal}, Caroline and Rozenn Souillard and Fabrizio Anniballi and Bruna Auricchio and Luca Bano and Bayon-Auboyer, {Marie H{\'e}l{\`e}ne} and Miriam Koene and Isabelle Mermoud and Brito, {Roseane B.} and Lobato, {Francisco C.F.} and Silva, {Rodrigo O.S.} and Dorner, {Martin B.} and Patrick Fach",

year = "2018",

month = feb,

day = "1",

doi = "10.1016/j.anaerobe.2017.12.009",

language = "English",

volume = "49",

pages = "71--77",

journal = "Anaerobe",

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Investigation of Clostridium botulinum group III's mobilome content. / Woudstra, Cédric; Le Maréchal, Caroline; Souillard, Rozenn; Anniballi, Fabrizio; Auricchio, Bruna; Bano, Luca; Bayon-Auboyer, Marie Hélène; Koene, Miriam; Mermoud, Isabelle; Brito, Roseane B.; Lobato, Francisco C.F.; Silva, Rodrigo O.S.; Dorner, Martin B.; Fach, Patrick.

In: Anaerobe, Vol. 49, 01.02.2018, p. 71-77.

Research output: Contribution to journal › Article › Academic › peer-review

TY - JOUR

T1 - Investigation of Clostridium botulinum group III's mobilome content

AU - Woudstra, Cédric

AU - Le Maréchal, Caroline

AU - Souillard, Rozenn

AU - Anniballi, Fabrizio

AU - Auricchio, Bruna

AU - Bano, Luca

AU - Bayon-Auboyer, Marie Hélène

AU - Koene, Miriam

AU - Mermoud, Isabelle

AU - Brito, Roseane B.

AU - Lobato, Francisco C.F.

AU - Silva, Rodrigo O.S.

AU - Dorner, Martin B.

AU - Fach, Patrick

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete C. botulinum group III genome sequence (strain BKT015925), strains have been found to contain others mobile elements encoding for toxin components. In this study, seven assays targeting toxin genes present on the genetic mobile elements of C. botulinum group III were developed with the objective to better characterize C. botulinum group III strains. The investigation of 110 C. botulinum group III strains and 519 naturally contaminated samples collected during botulism outbreaks in Europe showed alpha-toxin and C2-I/C2-II markers to be systematically associated with type C/D bont-positive samples, which may indicate an important role of these elements in the pathogenicity mechanisms. On the contrary, bont type D/C strains and the related positive samples appeared to contain almost none of the markers tested. Interestingly, 31 bont-negative samples collected on farms after a botulism outbreak revealed to be positive for some of the genetic mobile elements tested. This suggests loss of the bont phage, either in farm environment after the outbreak or during laboratory handling.

AB - Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete C. botulinum group III genome sequence (strain BKT015925), strains have been found to contain others mobile elements encoding for toxin components. In this study, seven assays targeting toxin genes present on the genetic mobile elements of C. botulinum group III were developed with the objective to better characterize C. botulinum group III strains. The investigation of 110 C. botulinum group III strains and 519 naturally contaminated samples collected during botulism outbreaks in Europe showed alpha-toxin and C2-I/C2-II markers to be systematically associated with type C/D bont-positive samples, which may indicate an important role of these elements in the pathogenicity mechanisms. On the contrary, bont type D/C strains and the related positive samples appeared to contain almost none of the markers tested. Interestingly, 31 bont-negative samples collected on farms after a botulism outbreak revealed to be positive for some of the genetic mobile elements tested. This suggests loss of the bont phage, either in farm environment after the outbreak or during laboratory handling.

KW - Animal botulism

KW - Clostridium botulinum group III

KW - Phage

KW - Plasmid

U2 - 10.1016/j.anaerobe.2017.12.009

DO - 10.1016/j.anaerobe.2017.12.009

M3 - Article

AN - SCOPUS:85039774300

VL - 49

SP - 71

EP - 77

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

ER -

Investigation of Clostridium botulinum group III's mobilome content

Abstract

Keywords

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Erratum to “Investigation of Clostridium botulinum group III's mobilome content”

Cite this