Investigation of Clostridium botulinum group III's mobilome content

Cédric Woudstra, Caroline Le Maréchal, Rozenn Souillard, Fabrizio Anniballi, Bruna Auricchio, Luca Bano, Marie Hélène Bayon-Auboyer, Miriam Koene, Isabelle Mermoud, Roseane B. Brito, Francisco C.F. Lobato, Rodrigo O.S. Silva, Martin B. Dorner, Patrick Fach*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

1 Citation (Scopus)

Abstract

Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete C. botulinum group III genome sequence (strain BKT015925), strains have been found to contain others mobile elements encoding for toxin components. In this study, seven assays targeting toxin genes present on the genetic mobile elements of C. botulinum group III were developed with the objective to better characterize C. botulinum group III strains. The investigation of 110 C. botulinum group III strains and 519 naturally contaminated samples collected during botulism outbreaks in Europe showed alpha-toxin and C2-I/C2-II markers to be systematically associated with type C/D bont-positive samples, which may indicate an important role of these elements in the pathogenicity mechanisms. On the contrary, bont type D/C strains and the related positive samples appeared to contain almost none of the markers tested. Interestingly, 31 bont-negative samples collected on farms after a botulism outbreak revealed to be positive for some of the genetic mobile elements tested. This suggests loss of the bont phage, either in farm environment after the outbreak or during laboratory handling.
Original languageEnglish
Pages (from-to)71-77
JournalAnaerobe
Volume49
DOIs
Publication statusPublished - 1 Feb 2018

Fingerprint

Clostridium botulinum
Botulism
Interspersed Repetitive Sequences
Disease Outbreaks
Bacteriophages
Dilatation and Curettage
Gene Targeting
Botulinum Toxins
Virulence
Economics
Genome
Mortality

Keywords

  • Animal botulism
  • Clostridium botulinum group III
  • Phage
  • Plasmid

Cite this

Woudstra, C., Le Maréchal, C., Souillard, R., Anniballi, F., Auricchio, B., Bano, L., ... Fach, P. (2018). Investigation of Clostridium botulinum group III's mobilome content. Anaerobe, 49, 71-77. https://doi.org/10.1016/j.anaerobe.2017.12.009
Woudstra, Cédric ; Le Maréchal, Caroline ; Souillard, Rozenn ; Anniballi, Fabrizio ; Auricchio, Bruna ; Bano, Luca ; Bayon-Auboyer, Marie Hélène ; Koene, Miriam ; Mermoud, Isabelle ; Brito, Roseane B. ; Lobato, Francisco C.F. ; Silva, Rodrigo O.S. ; Dorner, Martin B. ; Fach, Patrick. / Investigation of Clostridium botulinum group III's mobilome content. In: Anaerobe. 2018 ; Vol. 49. pp. 71-77.
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abstract = "Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete C. botulinum group III genome sequence (strain BKT015925), strains have been found to contain others mobile elements encoding for toxin components. In this study, seven assays targeting toxin genes present on the genetic mobile elements of C. botulinum group III were developed with the objective to better characterize C. botulinum group III strains. The investigation of 110 C. botulinum group III strains and 519 naturally contaminated samples collected during botulism outbreaks in Europe showed alpha-toxin and C2-I/C2-II markers to be systematically associated with type C/D bont-positive samples, which may indicate an important role of these elements in the pathogenicity mechanisms. On the contrary, bont type D/C strains and the related positive samples appeared to contain almost none of the markers tested. Interestingly, 31 bont-negative samples collected on farms after a botulism outbreak revealed to be positive for some of the genetic mobile elements tested. This suggests loss of the bont phage, either in farm environment after the outbreak or during laboratory handling.",
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author = "C{\'e}dric Woudstra and {Le Mar{\'e}chal}, Caroline and Rozenn Souillard and Fabrizio Anniballi and Bruna Auricchio and Luca Bano and Bayon-Auboyer, {Marie H{\'e}l{\`e}ne} and Miriam Koene and Isabelle Mermoud and Brito, {Roseane B.} and Lobato, {Francisco C.F.} and Silva, {Rodrigo O.S.} and Dorner, {Martin B.} and Patrick Fach",
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Woudstra, C, Le Maréchal, C, Souillard, R, Anniballi, F, Auricchio, B, Bano, L, Bayon-Auboyer, MH, Koene, M, Mermoud, I, Brito, RB, Lobato, FCF, Silva, ROS, Dorner, MB & Fach, P 2018, 'Investigation of Clostridium botulinum group III's mobilome content', Anaerobe, vol. 49, pp. 71-77. https://doi.org/10.1016/j.anaerobe.2017.12.009

Investigation of Clostridium botulinum group III's mobilome content. / Woudstra, Cédric; Le Maréchal, Caroline; Souillard, Rozenn; Anniballi, Fabrizio; Auricchio, Bruna; Bano, Luca; Bayon-Auboyer, Marie Hélène; Koene, Miriam; Mermoud, Isabelle; Brito, Roseane B.; Lobato, Francisco C.F.; Silva, Rodrigo O.S.; Dorner, Martin B.; Fach, Patrick.

In: Anaerobe, Vol. 49, 01.02.2018, p. 71-77.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Investigation of Clostridium botulinum group III's mobilome content

AU - Woudstra, Cédric

AU - Le Maréchal, Caroline

AU - Souillard, Rozenn

AU - Anniballi, Fabrizio

AU - Auricchio, Bruna

AU - Bano, Luca

AU - Bayon-Auboyer, Marie Hélène

AU - Koene, Miriam

AU - Mermoud, Isabelle

AU - Brito, Roseane B.

AU - Lobato, Francisco C.F.

AU - Silva, Rodrigo O.S.

AU - Dorner, Martin B.

AU - Fach, Patrick

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete C. botulinum group III genome sequence (strain BKT015925), strains have been found to contain others mobile elements encoding for toxin components. In this study, seven assays targeting toxin genes present on the genetic mobile elements of C. botulinum group III were developed with the objective to better characterize C. botulinum group III strains. The investigation of 110 C. botulinum group III strains and 519 naturally contaminated samples collected during botulism outbreaks in Europe showed alpha-toxin and C2-I/C2-II markers to be systematically associated with type C/D bont-positive samples, which may indicate an important role of these elements in the pathogenicity mechanisms. On the contrary, bont type D/C strains and the related positive samples appeared to contain almost none of the markers tested. Interestingly, 31 bont-negative samples collected on farms after a botulism outbreak revealed to be positive for some of the genetic mobile elements tested. This suggests loss of the bont phage, either in farm environment after the outbreak or during laboratory handling.

AB - Clostridium botulinum group III is mainly responsible for botulism in animals. It could lead to high animal mortality rates and, therefore, represents a major environmental and economic concern. Strains of this group harbor the botulinum toxin locus on an unstable bacteriophage. Since the release of the first complete C. botulinum group III genome sequence (strain BKT015925), strains have been found to contain others mobile elements encoding for toxin components. In this study, seven assays targeting toxin genes present on the genetic mobile elements of C. botulinum group III were developed with the objective to better characterize C. botulinum group III strains. The investigation of 110 C. botulinum group III strains and 519 naturally contaminated samples collected during botulism outbreaks in Europe showed alpha-toxin and C2-I/C2-II markers to be systematically associated with type C/D bont-positive samples, which may indicate an important role of these elements in the pathogenicity mechanisms. On the contrary, bont type D/C strains and the related positive samples appeared to contain almost none of the markers tested. Interestingly, 31 bont-negative samples collected on farms after a botulism outbreak revealed to be positive for some of the genetic mobile elements tested. This suggests loss of the bont phage, either in farm environment after the outbreak or during laboratory handling.

KW - Animal botulism

KW - Clostridium botulinum group III

KW - Phage

KW - Plasmid

U2 - 10.1016/j.anaerobe.2017.12.009

DO - 10.1016/j.anaerobe.2017.12.009

M3 - Article

VL - 49

SP - 71

EP - 77

JO - Anaerobe

JF - Anaerobe

SN - 1075-9964

ER -

Woudstra C, Le Maréchal C, Souillard R, Anniballi F, Auricchio B, Bano L et al. Investigation of Clostridium botulinum group III's mobilome content. Anaerobe. 2018 Feb 1;49:71-77. https://doi.org/10.1016/j.anaerobe.2017.12.009