Investigation into the potential of commercially available lesser mealworm (A. diaperinus) protein to serve as sources of peptides with DPP-IV inhibitory activity

Isabelle M.E. Lacroix*, Irene Dávalos Terán, Vincenzo Fogliano, Harry J. Wichers

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Food-derived peptides are known to possess inhibitory activity against the dipeptidyl-peptidase IV (DPP-IV) enzyme, a target in the management of type 2 diabetes. While proteins from commonly consumed food commodities have been investigated as precursors of DPP-IV-inhibiting peptides, studies on novel protein sources, such as those from insects, are sparse. This research aimed to determine if DPP-IV inhibitors can be generated upon in vitro digestion or enzymatic hydrolysis of lesser mealworm protein isolate and concentrate. Treatment of the proteins with digestive enzymes and proteases generated hydrolysates with varying potency, thermolysin being the most effective at releasing active peptides (IC50= 0.63 and 0.60 mg mL−1 for the isolate and concentrate). Ultrafiltration of the thermolysin-treated hydrolysates did not significantly improve the potency. This study shows that DPP-IV inhibitors can be generated from lesser mealworm protein and provides insight on the potential of insects to serve as functional food ingredients.

Original languageEnglish
Pages (from-to)696-704
Number of pages9
JournalInternational Journal of Food Science and Technology
Volume54
Issue number3
Early online date16 Oct 2018
DOIs
Publication statusPublished - Mar 2019

Fingerprint

Alphitobius diaperinus
Tenebrio
Dipeptidyl Peptidase 4
Peptides
peptides
Proteins
Dipeptidyl-Peptidase IV Inhibitors
Thermolysin
hydrolysates
proteins
Insects
commodity foods
Enzymes
insects
protein concentrates
protein isolates
digestive enzymes
ultrafiltration
enzymatic hydrolysis
Food

Keywords

  • Dipeptidyl-peptidase IV inhibitors
  • hydrolysates
  • in vitro digestion
  • lesser mealworm (A. diaperinus) protein
  • type 2 diabetes

Cite this

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title = "Investigation into the potential of commercially available lesser mealworm (A. diaperinus) protein to serve as sources of peptides with DPP-IV inhibitory activity",
abstract = "Food-derived peptides are known to possess inhibitory activity against the dipeptidyl-peptidase IV (DPP-IV) enzyme, a target in the management of type 2 diabetes. While proteins from commonly consumed food commodities have been investigated as precursors of DPP-IV-inhibiting peptides, studies on novel protein sources, such as those from insects, are sparse. This research aimed to determine if DPP-IV inhibitors can be generated upon in vitro digestion or enzymatic hydrolysis of lesser mealworm protein isolate and concentrate. Treatment of the proteins with digestive enzymes and proteases generated hydrolysates with varying potency, thermolysin being the most effective at releasing active peptides (IC50= 0.63 and 0.60 mg mL−1 for the isolate and concentrate). Ultrafiltration of the thermolysin-treated hydrolysates did not significantly improve the potency. This study shows that DPP-IV inhibitors can be generated from lesser mealworm protein and provides insight on the potential of insects to serve as functional food ingredients.",
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Investigation into the potential of commercially available lesser mealworm (A. diaperinus) protein to serve as sources of peptides with DPP-IV inhibitory activity. / Lacroix, Isabelle M.E.; Dávalos Terán, Irene; Fogliano, Vincenzo; Wichers, Harry J.

In: International Journal of Food Science and Technology, Vol. 54, No. 3, 03.2019, p. 696-704.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Lacroix, Isabelle M.E.

AU - Dávalos Terán, Irene

AU - Fogliano, Vincenzo

AU - Wichers, Harry J.

PY - 2019/3

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