Investigating the Transport Dynamics of Anthocyanins from Unprocessed Fruit and Processed Fruit Juice from Sour Cherry (Prunus cerasus L.) across Intestinal Epithelial Cells

G. Toydemir, D. Boyacioglu, E. Capanoglu, I.M. van der Meer, M.M.M. Tomassen, R.D. Hall, J.J. Mes, J. Beekwilder

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17 Citations (Scopus)

Abstract

Anthocyanins can contribute to human health through preventing a variety of diseases. The uptake of these compounds from food and the parameters determining uptake efficiency within the human body are still poorly understood. Here we have employed a Caco-2 cell based system to investigate the transport of key antioxidant food components from sour cherry (Prunus cerasus L.) across the intestinal epithelial barrier. Anthocyanins and (-)-epicatechin were supplied in three contrasting matrices: fruit, processed fruit cherry juice, and polyphenolic fractions obtained by solid-phase extraction. Results show that both compound types behave differently. Fruit or juice matrices display comparable transport across the epithelial cell layer. The juice supplements sucrose and citric acid, which are regularly added to processed foods, have a positive effect on stability and transport. Polyphenolic fractions display a lower transport efficiency, relative to that of the fruit or juice, indicating the importance of food matrix components for intestinal absorption of polyphenols
Original languageEnglish
Pages (from-to)11434-11441
JournalJournal of Agricultural and Food Chemistry
Volume61
Issue number47
DOIs
Publication statusPublished - 2013

Fingerprint

Fruit juices
Prunus cerasus
Anthocyanins
fruit juices
Fruits
anthocyanins
juices
Fruit
epithelial cells
Epithelial Cells
Food
polyphenols
fruits
Processed foods
Catechin
Polyphenols
uptake mechanisms
Citric Acid
Caco-2 Cells
Sucrose

Keywords

  • red grape juice
  • protein interactions
  • cellular uptake
  • absorption
  • quercetin
  • dietary
  • caco-2
  • bioavailability
  • consumption
  • glucosides

Cite this

@article{17eb36610d3341abae412202b4a86652,
title = "Investigating the Transport Dynamics of Anthocyanins from Unprocessed Fruit and Processed Fruit Juice from Sour Cherry (Prunus cerasus L.) across Intestinal Epithelial Cells",
abstract = "Anthocyanins can contribute to human health through preventing a variety of diseases. The uptake of these compounds from food and the parameters determining uptake efficiency within the human body are still poorly understood. Here we have employed a Caco-2 cell based system to investigate the transport of key antioxidant food components from sour cherry (Prunus cerasus L.) across the intestinal epithelial barrier. Anthocyanins and (-)-epicatechin were supplied in three contrasting matrices: fruit, processed fruit cherry juice, and polyphenolic fractions obtained by solid-phase extraction. Results show that both compound types behave differently. Fruit or juice matrices display comparable transport across the epithelial cell layer. The juice supplements sucrose and citric acid, which are regularly added to processed foods, have a positive effect on stability and transport. Polyphenolic fractions display a lower transport efficiency, relative to that of the fruit or juice, indicating the importance of food matrix components for intestinal absorption of polyphenols",
keywords = "red grape juice, protein interactions, cellular uptake, absorption, quercetin, dietary, caco-2, bioavailability, consumption, glucosides",
author = "G. Toydemir and D. Boyacioglu and E. Capanoglu and {van der Meer}, I.M. and M.M.M. Tomassen and R.D. Hall and J.J. Mes and J. Beekwilder",
year = "2013",
doi = "10.1021/jf4032519",
language = "English",
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pages = "11434--11441",
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TY - JOUR

T1 - Investigating the Transport Dynamics of Anthocyanins from Unprocessed Fruit and Processed Fruit Juice from Sour Cherry (Prunus cerasus L.) across Intestinal Epithelial Cells

AU - Toydemir, G.

AU - Boyacioglu, D.

AU - Capanoglu, E.

AU - van der Meer, I.M.

AU - Tomassen, M.M.M.

AU - Hall, R.D.

AU - Mes, J.J.

AU - Beekwilder, J.

PY - 2013

Y1 - 2013

N2 - Anthocyanins can contribute to human health through preventing a variety of diseases. The uptake of these compounds from food and the parameters determining uptake efficiency within the human body are still poorly understood. Here we have employed a Caco-2 cell based system to investigate the transport of key antioxidant food components from sour cherry (Prunus cerasus L.) across the intestinal epithelial barrier. Anthocyanins and (-)-epicatechin were supplied in three contrasting matrices: fruit, processed fruit cherry juice, and polyphenolic fractions obtained by solid-phase extraction. Results show that both compound types behave differently. Fruit or juice matrices display comparable transport across the epithelial cell layer. The juice supplements sucrose and citric acid, which are regularly added to processed foods, have a positive effect on stability and transport. Polyphenolic fractions display a lower transport efficiency, relative to that of the fruit or juice, indicating the importance of food matrix components for intestinal absorption of polyphenols

AB - Anthocyanins can contribute to human health through preventing a variety of diseases. The uptake of these compounds from food and the parameters determining uptake efficiency within the human body are still poorly understood. Here we have employed a Caco-2 cell based system to investigate the transport of key antioxidant food components from sour cherry (Prunus cerasus L.) across the intestinal epithelial barrier. Anthocyanins and (-)-epicatechin were supplied in three contrasting matrices: fruit, processed fruit cherry juice, and polyphenolic fractions obtained by solid-phase extraction. Results show that both compound types behave differently. Fruit or juice matrices display comparable transport across the epithelial cell layer. The juice supplements sucrose and citric acid, which are regularly added to processed foods, have a positive effect on stability and transport. Polyphenolic fractions display a lower transport efficiency, relative to that of the fruit or juice, indicating the importance of food matrix components for intestinal absorption of polyphenols

KW - red grape juice

KW - protein interactions

KW - cellular uptake

KW - absorption

KW - quercetin

KW - dietary

KW - caco-2

KW - bioavailability

KW - consumption

KW - glucosides

U2 - 10.1021/jf4032519

DO - 10.1021/jf4032519

M3 - Article

VL - 61

SP - 11434

EP - 11441

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 47

ER -