Intrusive growth of flax phloem fibers is of intercalary type

M. Ageeva, B. Petrovská, H. Kieft, V.V. Sal'nikov, A.V. Snegireva, J.E.G. van Dam, W.L.H. van Veenendaal, A.M.C. Emons, T.A. Gorshkova, A.A.M. van Lammeren

Research output: Contribution to journalArticleAcademicpeer-review

50 Citations (Scopus)

Abstract

Flax (Linum usitatissimum L.) phloem fibers elongate considerably during their development and intrude between existing cells. We questioned whether fiber elongation is caused by cell tip growth or intercalary growth. Cells with tip growth are characterized by having two specific zones of cytoplasm in the cell tip, one with vesicles and no large organelles at the very tip and one with various organelles amongst others longitudinally arranged cortical microtubules in the subapex. Such zones were not observed in elongating flax fibers. Instead, organelles moved into the very tip region, and cortical microtubules showed transversal and helical configurations as known for cells growing in intercalary way. In addition, pulse-chase experiments with Calcofluor White resulted in a spotted fluorescence in the cell wall all over the length of the fiber. Therefore, it is concluded that fiber elongation is not achieved by tip growth but by intercalary growth. The intrusively growing fiber is a coenocytic cell that has no plasmodesmata, making the fibers a symplastically isolated domain within the stem.
Original languageEnglish
Pages (from-to)565-574
JournalPlanta
Volume222
Issue number4
DOIs
Publication statusPublished - 2005

Fingerprint

Phloem
Flax
flax
phloem
Growth
Organelles
organelles
cells
Microtubules
microtubules
Plasmodesmata
plasmodesmata
Linum usitatissimum
Cell Wall
Cytoplasm
cytoplasm
Fluorescence
cell walls
fluorescence
stems

Keywords

  • arabidopsis root hairs
  • cell expansion
  • tip growth
  • cytoskeleton
  • plant
  • microtubules
  • polarity

Cite this

Ageeva, M. ; Petrovská, B. ; Kieft, H. ; Sal'nikov, V.V. ; Snegireva, A.V. ; van Dam, J.E.G. ; van Veenendaal, W.L.H. ; Emons, A.M.C. ; Gorshkova, T.A. ; van Lammeren, A.A.M. / Intrusive growth of flax phloem fibers is of intercalary type. In: Planta. 2005 ; Vol. 222, No. 4. pp. 565-574.
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abstract = "Flax (Linum usitatissimum L.) phloem fibers elongate considerably during their development and intrude between existing cells. We questioned whether fiber elongation is caused by cell tip growth or intercalary growth. Cells with tip growth are characterized by having two specific zones of cytoplasm in the cell tip, one with vesicles and no large organelles at the very tip and one with various organelles amongst others longitudinally arranged cortical microtubules in the subapex. Such zones were not observed in elongating flax fibers. Instead, organelles moved into the very tip region, and cortical microtubules showed transversal and helical configurations as known for cells growing in intercalary way. In addition, pulse-chase experiments with Calcofluor White resulted in a spotted fluorescence in the cell wall all over the length of the fiber. Therefore, it is concluded that fiber elongation is not achieved by tip growth but by intercalary growth. The intrusively growing fiber is a coenocytic cell that has no plasmodesmata, making the fibers a symplastically isolated domain within the stem.",
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author = "M. Ageeva and B. Petrovsk{\'a} and H. Kieft and V.V. Sal'nikov and A.V. Snegireva and {van Dam}, J.E.G. and {van Veenendaal}, W.L.H. and A.M.C. Emons and T.A. Gorshkova and {van Lammeren}, A.A.M.",
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Ageeva, M, Petrovská, B, Kieft, H, Sal'nikov, VV, Snegireva, AV, van Dam, JEG, van Veenendaal, WLH, Emons, AMC, Gorshkova, TA & van Lammeren, AAM 2005, 'Intrusive growth of flax phloem fibers is of intercalary type' Planta, vol. 222, no. 4, pp. 565-574. https://doi.org/10.1007/s00425-005-1536-2

Intrusive growth of flax phloem fibers is of intercalary type. / Ageeva, M.; Petrovská, B.; Kieft, H.; Sal'nikov, V.V.; Snegireva, A.V.; van Dam, J.E.G.; van Veenendaal, W.L.H.; Emons, A.M.C.; Gorshkova, T.A.; van Lammeren, A.A.M.

In: Planta, Vol. 222, No. 4, 2005, p. 565-574.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Intrusive growth of flax phloem fibers is of intercalary type

AU - Ageeva, M.

AU - Petrovská, B.

AU - Kieft, H.

AU - Sal'nikov, V.V.

AU - Snegireva, A.V.

AU - van Dam, J.E.G.

AU - van Veenendaal, W.L.H.

AU - Emons, A.M.C.

AU - Gorshkova, T.A.

AU - van Lammeren, A.A.M.

PY - 2005

Y1 - 2005

N2 - Flax (Linum usitatissimum L.) phloem fibers elongate considerably during their development and intrude between existing cells. We questioned whether fiber elongation is caused by cell tip growth or intercalary growth. Cells with tip growth are characterized by having two specific zones of cytoplasm in the cell tip, one with vesicles and no large organelles at the very tip and one with various organelles amongst others longitudinally arranged cortical microtubules in the subapex. Such zones were not observed in elongating flax fibers. Instead, organelles moved into the very tip region, and cortical microtubules showed transversal and helical configurations as known for cells growing in intercalary way. In addition, pulse-chase experiments with Calcofluor White resulted in a spotted fluorescence in the cell wall all over the length of the fiber. Therefore, it is concluded that fiber elongation is not achieved by tip growth but by intercalary growth. The intrusively growing fiber is a coenocytic cell that has no plasmodesmata, making the fibers a symplastically isolated domain within the stem.

AB - Flax (Linum usitatissimum L.) phloem fibers elongate considerably during their development and intrude between existing cells. We questioned whether fiber elongation is caused by cell tip growth or intercalary growth. Cells with tip growth are characterized by having two specific zones of cytoplasm in the cell tip, one with vesicles and no large organelles at the very tip and one with various organelles amongst others longitudinally arranged cortical microtubules in the subapex. Such zones were not observed in elongating flax fibers. Instead, organelles moved into the very tip region, and cortical microtubules showed transversal and helical configurations as known for cells growing in intercalary way. In addition, pulse-chase experiments with Calcofluor White resulted in a spotted fluorescence in the cell wall all over the length of the fiber. Therefore, it is concluded that fiber elongation is not achieved by tip growth but by intercalary growth. The intrusively growing fiber is a coenocytic cell that has no plasmodesmata, making the fibers a symplastically isolated domain within the stem.

KW - arabidopsis root hairs

KW - cell expansion

KW - tip growth

KW - cytoskeleton

KW - plant

KW - microtubules

KW - polarity

U2 - 10.1007/s00425-005-1536-2

DO - 10.1007/s00425-005-1536-2

M3 - Article

VL - 222

SP - 565

EP - 574

JO - Planta

JF - Planta

SN - 0032-0935

IS - 4

ER -