Introduction of ethoxy-resorufin-o-deethylase activity by halogenated aromatic hydrocarbons and polycyclic aromatic hydrocarbons in primary hepatocytes of the green frog (Rana esculenta)

T. Rouhani Rankouhi, B. Koomen, J.T. Sanderson, A.T.C. Bosveld, W. Seinen, M. van den Berg

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

In this study, we measured the ethoxy-resorufin-O-deethylase (EROD) activity in primary hepatocytes of the common green frog Rana esculenta as a biomarker for cytochrome P4501A induction. We exposed hepatocytes derived from male and female frogs to several halogenated aromatic hydrocarbons, such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachlorodibenzofuran (PCDF), polychlorinated biphenyls (PCB-126, PCB-118). and polycyclic aromatic hydrocarbons such as benzo[a]pyrene (BaP), chrysene, anthracene, and pyrene. Exposure to PCB-118, anthracene, and pyrene, up to 1 μ M, did not induce EROD activity, whereas TCDD and PCDF induced EROD activity maximally. In our primary frog hepatocytes, exposure to chrysene and BaP resulted in median effective concentration values (EC50) in the high nM range (82-1035 nM). Exposure to TCDD, PCDF and PCB-126 resulted in EC50 values of 0.4 to 8, 0.07 to 0.7, and 3 to 133 nM, respectively, which is in the same range as EC50 values found in primary hepatocytes of birds. Compared to our frog hepatocytes, primary rat hepatocytes seem to be more sensitive to TCDD, chrysene. and BaP.
Original languageEnglish
Pages (from-to)1428-1435
JournalEnvironmental Toxicology and Chemistry
Volume24
Issue number6
Publication statusPublished - 2005

Keywords

  • polychlorinated-biphenyls pcbs
  • cell-line
  • in-vitro
  • cytochrome-p4501a induction
  • endocrine disruptors
  • population declines
  • xenopus-laevis
  • fish
  • cultures
  • liver

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