Introduction of beet cyst nematode resistance from Sinapis alba L. and Raphanus sativus L. into Brassica napus L. (oil-seed rape) through sexual and somatic hybridization

C.L.C. Lelivelt

Research output: Thesisexternal PhD, WU

Abstract

Experiments were performed to select for beet cyst nematode (Heterodera schachtii Schm., abbrev. BCN) resistant genotypes of Brassica napus L. (oilseed rape), and to introduce BCN-resistance from the related species Raphanus sativus L. (oil-radish) and Sinapis alba L. (white mustard) into oil-seed rape.

Sexual hybridization between B.napus and R. sativus did not result in hybrid plants, whereas from about 800 crosses between B.napus and the amphidiploid xBrassicoraphanus Sageret 284 F 1 hybrid plants were obtained. Sexual hybridization between B.napus and S. alba was only successful when diploid accessions of S.alba were used as the female parent. Crossability between these species was poor; only six hybrids were obtained out of approximately 10,000 crosses. The poor crossability in the intergeneric crosses was shown to be the cause of various breeding barriers. Somatic hybridization between B.napus and either R. sativus or S. alba resulted in a few somatic hybrid plants. Putative F 1 hybrids and somatic hybrid plants were characterized by their morphology, cytology, by DNA-analysis and by scoring resistance to BCN. Somatic hybrid plants were found to be unstable for the number of chromosomes and for BCN- resistance. Some F 1 hybrids, somatic hybrids and BC 1 plants, derived from crossing F 1 hybrids to B.napus as male parent had a high level of BCN-resistance, not different from that of the resistant parental genotypes. Finally, the mechanism of resistance to BCN in resistant S. alba, R. sativus and xBrassico-raphanus was expressed in F 1 hybrids derived from crosses between resistant genotypes of these three species and B. napus.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Hermsen, J.G.T., Promotor, External person
Award date30 Mar 1993
Place of PublicationWageningen
Publisher
Publication statusPublished - 30 Mar 1993

Keywords

  • raphanus sativus
  • radishes
  • brassica napus var. oleifera
  • rape
  • somatic hybridization
  • interspecific hybridization
  • hybrids
  • mustard
  • plant pests
  • pratylenchus
  • heteroderidae
  • tylenchidae
  • plant breeding
  • disease resistance
  • pest resistance

Fingerprint

Dive into the research topics of 'Introduction of beet cyst nematode resistance from Sinapis alba L. and Raphanus sativus L. into Brassica napus L. (oil-seed rape) through sexual and somatic hybridization'. Together they form a unique fingerprint.

Cite this