Introduction, maintenance and utilization of male sterility in leek (Allium ampeloprasum L.) = [Inductie, handhaving en gebruik van mannelijke steriliteit in prei (Allium ampeloprasum L.)]

B. Silvertand

Research output: Thesisinternal PhD, WU

Abstract

<br/>This thesis describes research work performed in leek in relation to efforts to obtain or exploit a male sterility system for hybrid leek breeding. Male sterile plants can be found in every leek cultivar. The male sterility trait can be explained by a nuclear monogenic inheritance or by a nucleo-cytoplasmic inheritance. The existence of a nucleo-cytoplasmic inheritance remains speculative unless the maintainer genotype will be identified. No source of CMS has been found, sofar, in leek or related forms of <em>A. ampeloprasum</em> L.<p>Mutation breeding experiments during an extended period of time, using chemicals or somaclonal variation, did not result in the induction of cytoplasmic male sterile mutants in leek.<p>The small chances for fixation of a mutation in a mitochondrial genome population and problems concerning the identification and recovery of the cytoplasmic mutation at the plant level may have been the major obstacles in obtaining a cytoplasmic male sterile mutant.<p>An efficient callus regeneration system and the selection of leek plants with improved regeneration ability are described in this thesis. The availability of a regeneration system is an essential step in the application of somatic hybridization, somaclonal variation and transformation techniques in leek.<p>Vegetative propagation of nuclear male sterile genotypes remains up till now the most realistic option for producing leek hybrids. A new <em>in vitro</em> method for mass clonal propagation of leek, based on flower stalk explants, was developed and is described in this thesis. This method offers a realistic opportunity to leek breeders for producing leek hybrids, until a less expensive genetic system for producing male sterile lines would become available.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Jacobsen, Evert, Promotor
  • van Harten, A.M., Promotor, External person
Award date12 Apr 1996
Place of PublicationS.l.
Publisher
Print ISBNs9789054855125
Publication statusPublished - 1996

Keywords

  • allium ampeloprasum
  • allium porrum
  • leeks
  • male sterility
  • targeted mutagenesis

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