Intraspecific comparison of the mitochondrial genome sequences from two strains of the plant pathogenic fungus Mycosphaerella graminicola

S.F.F. Torriani, S.B. Goodwin, G.H.J. Kema, B.A. McDonald

    Research output: Contribution to journalArticleAcademicpeer-review

    51 Citations (Scopus)

    Abstract

    The mitochondrial genomes of two isolates of the wheat pathogen Mycosphaerella graminicola were sequenced completely and compared to identify polymorphic regions. This organism is of interest because it is phylogenetically distant from other fungi with sequenced mitochondrial genomes and it has shown discordant patterns of nuclear and mitochondrial diversity. The mitochondrial genome of M. graminicola is a circular molecule of approximately 43,960 bp containing the typical genes coding for 14 proteins related to oxidative phosphorylation, one RNA polymerase, two rRNA genes and a set of 27 tRNAs. The mitochondrial DNA of M. graminicola lacks the gene encoding the putative ribosomal protein (rps5-like), commonly found in fungal mitochondrial genomes. Most of the tRNA genes were clustered with a gene order conserved with many other ascomycetes. A sample of 35 additional strains representing the known global mt diversity was partially sequenced to measure overall mitochondrial variability within the species. Little variation was found, confirming previous RFLP-based findings of low mitochondrial diversity. The mitochondrial sequence of M. graminicola is the first reported from the family Mycosphaerellaceae or the order Capnodiales. The sequence also provides a tool to better understand the development of fungicide resistance and the conflicting pattern of high nuclear and low mitochondrial diversity in global populations of this fungus.
    Original languageEnglish
    Pages (from-to)628-637
    JournalFungal Genetics and Biology
    Volume45
    Issue number5
    DOIs
    Publication statusPublished - 2008

    Keywords

    • fragment-length-polymorphism
    • septoria-tritici blotch
    • complete dna-sequence
    • genetic-structure
    • nuclear
    • diversity
    • wheat
    • populations
    • evolution
    • selection

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