Intramuscular inoculation of calves with an experimental Newcastle disease virus-based vector vaccine elicits neutralizing antibodies against Rift Valley fever virus

Research output: Contribution to journalArticleAcademicpeer-review

40 Citations (Scopus)

Abstract

In the past decade, the use of Newcastle disease virus (NDV) as a vaccine vector for the prevention of economically important livestock diseases as well as for human diseases has been extensively explored. In this study, we have constructed a recombinant NDV vaccine virus, named NDFL-Gn, that produces the Rift Valley fever virus (RVFV) Gn glycoprotein. Calves were immunized via either the intranasal route or the intramuscular route. Delivery via the intranasal route elicited no detectable antibody responses, whereas delivery via the intramuscular route elicited antibodies against both NDV and the Gn protein. The RVFV-neutralizing activity of the antisera from intramuscularly vaccinated calves was demonstrated, suggesting that NDV is a promising vaccine vector for the prevention of RVF in calves.
Original languageEnglish
Pages (from-to)2271-2276
JournalVaccine
Volume28
Issue number11
DOIs
Publication statusPublished - 2010

Keywords

  • avian influenza
  • respiratory-tract
  • fusion protein
  • foreign gene
  • i interferon
  • cloned cdna
  • expression
  • humans
  • immunization
  • replication

Fingerprint Dive into the research topics of 'Intramuscular inoculation of calves with an experimental Newcastle disease virus-based vector vaccine elicits neutralizing antibodies against Rift Valley fever virus'. Together they form a unique fingerprint.

Cite this