Abstract
AIM: To determine the immunomodulatory effect of
Shiitake (a mushroom extract), we tested its effect
on liver-mediated immune regulation in a model of
immune-mediated colitis.
METHODS: Four groups of mice were studied. Colitis
was induced by intracolonic instillation of TNBS in
groups A and B. Groups A and C were treated daily
with Shiitake extract, while groups B and D received
bovine serum albumin. Mice were evaluated for
development of macroscopic and microscopic. The
immune effects of Shiitake were determined by FACS
analysis of intra-hepatic and intrasplenic lymphocytes
and IFN-¿ ELISPOT assay.
RESULTS: Administration of Shiitake resulted in
by an increased intrasplenic/intrahepatic CD4/CD8
lymphocyte ratio. These effects were accompanied by
a 17% increase in the number of intrahepatic natural
killer T (NKT) cells. A similar effect was observed when
Shiitake was administered to animals without disease
induction.
CONCLUSION: Shiitake extract affected livermediated
immune regulation by altering the NKT
lymphocyte distribution and increasing intrahepatic
CD8+ T lymphocyte trapping, thereby leading to
alleviation of immune-mediated colitis
marked alleviation of colitis, manifested by significant
improvement in the macroscopic and microscopic
scores, and by reduction in IFN-¿-producing colonies
in group A, compared to group B mice (1.5 pfu/mL vs
3.7 pfu/mL, respectively). This beneficial effect was
associated with a significant increase in the intrahepatic
CD8+ lymphocyte trapping, demonstrated
Original language | English |
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Pages (from-to) | 3872-3878 |
Journal | World Journal of Gastroenterology |
Volume | 14 |
Issue number | 24 |
DOIs | |
Publication status | Published - 2008 |
Keywords
- inflammatory-bowel-disease
- lentinus-edodes mycelia
- t-cells
- nkt cells
- experimental colitis
- medicinal mushrooms
- immune-reactions
- polysaccharides
- antitumor
- immunomodulation