Intragenic vectors for gene transfer without foreign DNA

A.J. Conner, P.J. Barrell, S.J. Baldwin, A.S. Lokerse, P.A. Cooper, A.K. Erasmuson, J.P.H. Nap, J.M.E. Jacobs

Research output: Contribution to journalArticleAcademicpeer-review

41 Citations (Scopus)


The intragenic vector system involves identifying functional equivalents of vector components from the genome of a specific crop species (or related species to which it can be hybridised) and using these DNA sequences to assemble vectors for transformation of that plant species. This system offers an attractive alternative to current genetic engineering strategies where vectors are based on DNA sequences that usually originate from bacteria. The construction of intragenic vectors enables the well-defined genetic improvement of plants with all transferred DNA originating from within the gene pool already available to plant breeders. In this manner genes can be introgressed into elite cultivars in a single step without linkage drag and without the incorporation of foreign DNA. The resulting plants are non-transgenic, although they are derived using the tools of molecular biology and plant transformation. The use of intragenic vectors for the transfer of genes from within the gene pools of crops may help to alleviate some of the major public concerns over the deployment of GM crops in agriculture, notably the ethical issue associated with the transfer of DNA across wide taxonomic boundaries. This paper reviews the progress toward the development and use of intragenic vectors and the implications of their use for the genetic improvement of crops.
Original languageEnglish
Pages (from-to)341-353
Issue number3
Publication statusPublished - 2007


  • agrobacterium-mediated transformation
  • minimal t-dna
  • complete nucleotide-sequence
  • genetically-modified crops
  • tumor-inducing ti
  • transgenic plants
  • backbone sequences
  • binary vector
  • repressor titration
  • cisgenic plants

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    Conner, A. J., Barrell, P. J., Baldwin, S. J., Lokerse, A. S., Cooper, P. A., Erasmuson, A. K., Nap, J. P. H., & Jacobs, J. M. E. (2007). Intragenic vectors for gene transfer without foreign DNA. Euphytica, 154(3), 341-353.