Intra- and interindividual variation in gene expression in human adipose tissue

E.A. van Beek, A.H. Bakker, P.M. Kruyt, M.H. Hofker, W.H.M. Saris, J. Keijer

    Research output: Contribution to journalArticleAcademicpeer-review

    35 Citations (Scopus)

    Abstract

    Adipose tissue is a highly plastic tissue with an important endocrine and metabolic function. To understand its role in human health and disease, it is necessary to understand the extent of variation and the specific differences within and between different depots and subjects. We employed cDNA microarray analysis to investigate this in human subjects ranging from lean to mildly obese. We observe (1) high similarity between different samples of one adipose depot, (2) only small differences between the subcutaneous and visceral adipose tissue depot and (3) larger differences in gene expression between different individuals (per depot). The major variation within adipose depots can be attributed to differences in the non-adipocyte component of adipose tissue. Using only non-obese subjects, we identified genes that were consistently differentially expressed between subcutaneous and omental adipose tissue, despite the variation in gene expression between these subjects. Using quantitative real time polymerase chain reaction (PCR), fatty acid binding protein 4 (FABP4), vimentin (Vim), four and a half LIMs domains (FHL1), CD36 (all higher in subcutaneous adipose tissue) and Matrix Gla protein (MGP; lower in subcutaneous adipose tissue) were confirmed to be significantly differentially expressed between depots
    Original languageEnglish
    Pages (from-to)851-861
    JournalPflugers Archiv-European Journal of Physiology
    Volume453
    Issue number6
    DOIs
    Publication statusPublished - 2007

    Keywords

    • matrix gla protein
    • depot-specific differences
    • messenger-rna expression
    • retinoic-acid
    • developmental expression
    • ribosomal-proteins
    • epithelial-cells
    • obese subjects
    • adipocytes
    • fat

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