Intestinal Uptake of Quercetin-3- Glucoside in Rats Involves Hydrolysis by Lactase Phlorizin Hydrolase

A.L.A. Sesink, I.C.W. Arts, M. Faassen-Peters, P.C.H. Hollman

Research output: Contribution to journalArticleAcademicpeer-review

76 Citations (Scopus)

Abstract

Quercetin has antioxidant, anti-inflammatory, antiproliferative and anticarcinogenic properties. In plant foods, quercetin occurs mainly bound to various sugars via a ß-glycosidic link. We hypothesized that lactase phlorizin hydrolase (LPH), an enzyme at the brush border membrane of intestinal cells, is involved in the in vivo intestinal uptake of quercetin-sugars. To study this, we measured the appearance of quercetin metabolites in plasma and perfusate after perfusing the jejunum and ileum with 50 µmol/L quercetin-3-glucoside in an in situ rat perfusion model. LPH was inhibited by the selective LPH inhibitor N-butyldeoxygalactonojirimycin (0, 0.5, 2 or 10 mmol/L) (n = 5 rats/group). Quercetin in plasma and perfusion buffer was determined by HPLC with CoulArray detection. Results are given as means ± SEM. In the perfusion buffer, 13.8 ± 0.7 µmol/L quercetin-3-glucoside was hydrolyzed during intestinal passage. Co-perfusion with 0.5, 2 and 10 mmol/L N-butyldeoxygalactonojirimycin resulted in 38% (P <0.05), 50% (P <0.01) and 67% (P <0.01) less hydrolysis, respectively. Plasma concentrations of quercetin in the corresponding groups were 36% (P = 0.12), 55% (P <0.01) and 75% (P <0.01) lower than in controls (1.23 ± 0.22 µmol/L). These data suggest that LPH is a major determinant of intestinal absorption of quercetin-3-glucoside in rats
Original languageEnglish
Pages (from-to)773-776
JournalThe Journal of Nutrition
Volume133
Issue number3
DOIs
Publication statusPublished - Mar 2003

Keywords

  • flavonoid quercetin 4'-beta-glucoside
  • beta-glucosidase activity
  • phlorhizin hydrolase
  • dietary quercetin
  • transport pathway
  • glycosides
  • absorption
  • consumption
  • plasma
  • sglt1

Fingerprint Dive into the research topics of 'Intestinal Uptake of Quercetin-3- Glucoside in Rats Involves Hydrolysis by Lactase Phlorizin Hydrolase'. Together they form a unique fingerprint.

  • Cite this