Intestinal uptake of genistein and its glycoside in the rat using various isolated perfused gut segments

A. Steensma, M.E. Ploum, H.P.J.M. Noteborn

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Genistein receives much attention because of its potential to prevent hormone-related cancer and cardiovascular diseases. Limited information is available on the pharmacokinetics of this compound like, for instance, their intestinal uptake by humans and systematic bioavailability. In this study, the fate of the absorption of genistein and its glycoside has been analysed in various isolated perfused gut segments of the rat. In all perfused gut segments the transport of genistein was higher compared to its glycoside. Furthermore, it appeared that the resorbate (i.e. serosal side) concentration of genistein was the highest in ileac segments, whereas the transport of genistein in the various other segments tested showed no difference between intestinal compartments. Less than 0.2 f genistin appeared in the resorbate fluid of all isolated gut segments. The main site of metabolism of genistein and its glycoside appears to be located in the jejunal compartment of the rat gut. About 38 f genistein and about 29 f genistin metabolised within 2 h of perfusion. In the ileac and colonic intestinal segments, genistein metabolised for only 10&Eth;For the first time, this study demonstrated that genistin could be metabolised by epithelial cells present in isolated colonic segments. However, the metabolites of genistin did not occur at the serosal side (the resorbate) of isolated colonic segments. We assume that there is no absorption of genistin and/or its metabolites in or through colonic tissue of the rat.
LanguageEnglish
Pages103-110
JournalEnvironmental Toxicology and Pharmacology
Volume17
Issue number2
DOIs
Publication statusPublished - 2004

Fingerprint

Genistein
Glycosides
Rats
Metabolites
Time and motion study
Pharmacokinetics
Time and Motion Studies
Metabolism
Biological Availability
Cardiovascular Diseases
Perfusion
Epithelial Cells
genistin
Hormones
Tissue
Fluids

Keywords

  • epithelial caco-2 cells
  • human plasma
  • quercetin
  • glucosides
  • bioavailability
  • absorption
  • women
  • quercetin-3-glucoside
  • isoflavones
  • transport

Cite this

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abstract = "Genistein receives much attention because of its potential to prevent hormone-related cancer and cardiovascular diseases. Limited information is available on the pharmacokinetics of this compound like, for instance, their intestinal uptake by humans and systematic bioavailability. In this study, the fate of the absorption of genistein and its glycoside has been analysed in various isolated perfused gut segments of the rat. In all perfused gut segments the transport of genistein was higher compared to its glycoside. Furthermore, it appeared that the resorbate (i.e. serosal side) concentration of genistein was the highest in ileac segments, whereas the transport of genistein in the various other segments tested showed no difference between intestinal compartments. Less than 0.2 f genistin appeared in the resorbate fluid of all isolated gut segments. The main site of metabolism of genistein and its glycoside appears to be located in the jejunal compartment of the rat gut. About 38 f genistein and about 29 f genistin metabolised within 2 h of perfusion. In the ileac and colonic intestinal segments, genistein metabolised for only 10&Eth;For the first time, this study demonstrated that genistin could be metabolised by epithelial cells present in isolated colonic segments. However, the metabolites of genistin did not occur at the serosal side (the resorbate) of isolated colonic segments. We assume that there is no absorption of genistin and/or its metabolites in or through colonic tissue of the rat.",
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author = "A. Steensma and M.E. Ploum and H.P.J.M. Noteborn",
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Intestinal uptake of genistein and its glycoside in the rat using various isolated perfused gut segments. / Steensma, A.; Ploum, M.E.; Noteborn, H.P.J.M.

In: Environmental Toxicology and Pharmacology, Vol. 17, No. 2, 2004, p. 103-110.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Intestinal uptake of genistein and its glycoside in the rat using various isolated perfused gut segments

AU - Steensma, A.

AU - Ploum, M.E.

AU - Noteborn, H.P.J.M.

PY - 2004

Y1 - 2004

N2 - Genistein receives much attention because of its potential to prevent hormone-related cancer and cardiovascular diseases. Limited information is available on the pharmacokinetics of this compound like, for instance, their intestinal uptake by humans and systematic bioavailability. In this study, the fate of the absorption of genistein and its glycoside has been analysed in various isolated perfused gut segments of the rat. In all perfused gut segments the transport of genistein was higher compared to its glycoside. Furthermore, it appeared that the resorbate (i.e. serosal side) concentration of genistein was the highest in ileac segments, whereas the transport of genistein in the various other segments tested showed no difference between intestinal compartments. Less than 0.2 f genistin appeared in the resorbate fluid of all isolated gut segments. The main site of metabolism of genistein and its glycoside appears to be located in the jejunal compartment of the rat gut. About 38 f genistein and about 29 f genistin metabolised within 2 h of perfusion. In the ileac and colonic intestinal segments, genistein metabolised for only 10&Eth;For the first time, this study demonstrated that genistin could be metabolised by epithelial cells present in isolated colonic segments. However, the metabolites of genistin did not occur at the serosal side (the resorbate) of isolated colonic segments. We assume that there is no absorption of genistin and/or its metabolites in or through colonic tissue of the rat.

AB - Genistein receives much attention because of its potential to prevent hormone-related cancer and cardiovascular diseases. Limited information is available on the pharmacokinetics of this compound like, for instance, their intestinal uptake by humans and systematic bioavailability. In this study, the fate of the absorption of genistein and its glycoside has been analysed in various isolated perfused gut segments of the rat. In all perfused gut segments the transport of genistein was higher compared to its glycoside. Furthermore, it appeared that the resorbate (i.e. serosal side) concentration of genistein was the highest in ileac segments, whereas the transport of genistein in the various other segments tested showed no difference between intestinal compartments. Less than 0.2 f genistin appeared in the resorbate fluid of all isolated gut segments. The main site of metabolism of genistein and its glycoside appears to be located in the jejunal compartment of the rat gut. About 38 f genistein and about 29 f genistin metabolised within 2 h of perfusion. In the ileac and colonic intestinal segments, genistein metabolised for only 10&Eth;For the first time, this study demonstrated that genistin could be metabolised by epithelial cells present in isolated colonic segments. However, the metabolites of genistin did not occur at the serosal side (the resorbate) of isolated colonic segments. We assume that there is no absorption of genistin and/or its metabolites in or through colonic tissue of the rat.

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KW - human plasma

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KW - glucosides

KW - bioavailability

KW - absorption

KW - women

KW - quercetin-3-glucoside

KW - isoflavones

KW - transport

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DO - 10.1016/j.etap.2004.03.008

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JO - Environmental Toxicology and Pharmacology

T2 - Environmental Toxicology and Pharmacology

JF - Environmental Toxicology and Pharmacology

SN - 1382-6689

IS - 2

ER -