Fluorescence in situ hybridization and real-time PCR analysis targeting the 16S rRNA gene of Akkermansia muciniphila were performed to determine its presence in the human intestinal tract. These techniques revealed that an A. muciniphila-like bacterium is a common member of the human intestinal tract and that its colonization starts in early life and develops within a year to a level close to that observed in adults (10(8) cells/g) but decreases (P <0.05) in the elderly.
- 16s ribosomal-rna
- human fecal samples
Collado, M. C., Derrien, M. M. N., Isolauri, E., de Vos, W. M., & Salminen, S. (2007). Intestinal integrity and Akkermansia muciniphila: a mucin-degrading member of the intestinal microbiota present in infants, adults and elderly. Applied and Environmental Microbiology, 73(23), 7767-7770. https://doi.org/10.1128/AEM.01477-07