Interplay between folding and assembly of fibril-forming polypeptides

R. Ni, S. Abeln, M. Schor, M.A. Cohen Stuart, P.G. Bolhuis

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)

Abstract

Polypeptides can self-assemble into hierarchically organized fibrils consisting of a stack of individually folded polypeptides driven together by hydrophobic interaction. Using a coarse-grained model, we systematically studied this self-assembly as a function of temperature and hydrophobicity of the residues on the outside of the building block. We find the self-assembly can occur via two different pathways-a random aggregation-folding route and a templated-folding process-thus indicating a strong coupling between folding and assembly. The simulation results can explain experimental evidence that assembly through stacking of folded building blocks is rarely observed, at the experimental concentrations. The model thus provides a generic picture of hierarchical fibril formation
Original languageEnglish
Article number058101
Number of pages5
JournalPhysical Review Letters
Volume111
Issue number5
DOIs
Publication statusPublished - 2013

Keywords

  • protein
  • simulations
  • disease

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