Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus

S.F. Hurst, S.E. Kidd, C.O. Morrissey, E. Snelders, W.J.G. Melchers, M.V. Castelli, E. Mellado, K. Simmon, C.A. Petti, S. Richardson, S. Zhang, A.M. Romanelli, B.L. Wickes, H.A. De Valk, C.H.W. Klaassen, S. Arunmozhi Balajee*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Seven international laboratories tested the recently proposed single-locus typing strategy for Aspergillus fumigatus subtyping for interlaboratory reproducibility. Comparative sequence analyses of portions of the locus AFUA-3G08990, encoding a putative cell surface protein (denoted CSP), was performed with a panel of Aspergillus isolates. Each laboratory followed very different protocols for extraction of DNA, PCR, and sequencing. Results revealed that the CSP typing method was a reproducible and portable strain typing method.

Original languageEnglish
Pages (from-to)1562-1564
Number of pages3
JournalJournal of Clinical Microbiology
Volume47
Issue number5
DOIs
Publication statusPublished - 1 May 2009
Externally publishedYes

Fingerprint

Dive into the research topics of 'Interlaboratory reproducibility of a single-locus sequence-based method for strain typing of Aspergillus fumigatus'. Together they form a unique fingerprint.

Cite this