Interlaboratory exercise for the analysis of carotenoids and related compounds in dried mango fruit (Mangifera indica L.)

José Villacís-Chiriboga, Griet Jacobs, John Van Camp, Kathy Elst, Jenny Ruales, Verónica Marcillo-Parra, Volker Böhm, Andrea Bunea, Martina Cirlini, Neal Craft, Bruno De Meulenaer, M.G. Dias, Giacomo Lazzarino, Antonio J. Meléndez-Martínez, Pieter Versloot, Adriana Z. Mercadante, Begoña Olmedilla-Alonso, Johana Ortiz-Ulloa, Carla M. Stinco, Stefan Voorspoels*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review


An interlaboratory comparison was done for the analysis of carotenoids in freeze-dried mango. The study was performed from July to September 2018. Mango fruit was freeze-dried, homogenized, and packaged under vacuum conditions in portions of 6 g (test sample). Two test samples were sent to the participating laboratories for analysis. Laboratory results were rated using Z-scores in accordance with ISO 13528 and ISO 17043. The standard deviation for proficiency assessment (also called target standard deviation) was determined using a modified Horwitz function and varied between 10% and 25%, depending on the analyte. Out of 14 laboratories from 10 different countries, 9 laboratories (64%) obtained a satisfactory performance (Z ≤ 2) for the analysis of β-carotene. While for 7 laboratories that analyzed α-carotene, (9Z)-β-carotene, β-cryptoxanthin, and zeaxanthin, 4 laboratories (57%) obtained a satisfactory performance. However, only 2 laboratories out of 7 (29%) obtained a satisfactory performance for lutein. Based on the comparability of the analytical results, this study concludes that freeze-dried mango pulp can be used as a reference material for the analysis of α and β-carotene, (9Z)-β-carotene, β-cryptoxanthin, and zeaxanthin by applying different analytical procedures for their extraction and quantification.

Original languageEnglish
Article number104616
JournalJournal of Food Composition and Analysis
Publication statusPublished - Aug 2022


  • Interlaboratory analysis
  • Isomers
  • Lipophilic compounds
  • Liquid chromatography
  • Quality assurance
  • Reference material


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