Interfacial behaviour of proteins, with special reference to immunoglobulins. A physicochemical study

W. Norde, J. Lyklema

Research output: Contribution to journalArticleAcademicpeer-review

42 Citations (Scopus)

Abstract

Some basic elements of the adsorption of proteins on solid surfaces are briefly reviewed, emphasizing immunoglobulins. The paper focuses on the physicochemical interactions and considers the precautions that have to be taken to let the protein adsorb in a way in which it is biologically active. Contributing factors include surface pretreatment, composition of the solution, (pH, nature and concentration of electrolytes, etc.), extent of reversibility, and lateral interactions in the adsorbed state. Particular attention is paid to the option of partially pre-coating the adsorbent by irreversibly adsorbed polymers to induce the later adsorbing immune globulin molecules to assume a biologically preferred orientation and conformation.
Original languageEnglish
Pages (from-to)5-13
JournalAdvances in Colloid and Interface Science
Volume179-182
DOIs
Publication statusPublished - 2012

Keywords

  • bovine pancreas ribonuclease
  • ultrafine silica particles
  • solid-liquid interfaces
  • human-plasma albumin
  • circular-dichroism
  • oriented immobilization
  • conformational-changes
  • polystyrene surfaces
  • adsorption
  • model

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