Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall

J.F.P. Timmers, S. Vernhettes, T. Desprez, J.P. Vincken, R.G.F. Visser, L.M. Trindade

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

It has not yet been reported how the secondary CESA (cellulose synthase) proteins are organized in the rosette structure. A membrane-based yeast two-hybrid (MbYTH) approach was used to analyze the interactions between the CESA proteins involved in secondary cell wall synthesis of Arabidopsis and the findings were confirmed in planta by bimolecular fluorescence complementation (BiFC) assay. Results indicated that although all CESA proteins can interact with each other, only CESA4 is able to form homodimers. A model is proposed for the secondary rosette structure. The RING-motif proved not to be essential for the interaction between the CESA proteins
Original languageEnglish
Pages (from-to)978-982
JournalFEBS Letters
Volume583
Issue number6
DOIs
Publication statusPublished - 2009

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Cell Wall
Cells
Membranes
Proteins
Arabidopsis
Yeast
Assays
Yeasts
Fluorescence
cellulose synthase

Keywords

  • arabidopsis
  • proteins
  • genes
  • identification
  • biosynthesis
  • domains
  • system
  • plants
  • cdna

Cite this

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abstract = "It has not yet been reported how the secondary CESA (cellulose synthase) proteins are organized in the rosette structure. A membrane-based yeast two-hybrid (MbYTH) approach was used to analyze the interactions between the CESA proteins involved in secondary cell wall synthesis of Arabidopsis and the findings were confirmed in planta by bimolecular fluorescence complementation (BiFC) assay. Results indicated that although all CESA proteins can interact with each other, only CESA4 is able to form homodimers. A model is proposed for the secondary rosette structure. The RING-motif proved not to be essential for the interaction between the CESA proteins",
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Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall. / Timmers, J.F.P.; Vernhettes, S.; Desprez, T.; Vincken, J.P.; Visser, R.G.F.; Trindade, L.M.

In: FEBS Letters, Vol. 583, No. 6, 2009, p. 978-982.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Interactions between membrane-bound cellulose synthases involved in the synthesis of the secondary cell wall

AU - Timmers, J.F.P.

AU - Vernhettes, S.

AU - Desprez, T.

AU - Vincken, J.P.

AU - Visser, R.G.F.

AU - Trindade, L.M.

PY - 2009

Y1 - 2009

N2 - It has not yet been reported how the secondary CESA (cellulose synthase) proteins are organized in the rosette structure. A membrane-based yeast two-hybrid (MbYTH) approach was used to analyze the interactions between the CESA proteins involved in secondary cell wall synthesis of Arabidopsis and the findings were confirmed in planta by bimolecular fluorescence complementation (BiFC) assay. Results indicated that although all CESA proteins can interact with each other, only CESA4 is able to form homodimers. A model is proposed for the secondary rosette structure. The RING-motif proved not to be essential for the interaction between the CESA proteins

AB - It has not yet been reported how the secondary CESA (cellulose synthase) proteins are organized in the rosette structure. A membrane-based yeast two-hybrid (MbYTH) approach was used to analyze the interactions between the CESA proteins involved in secondary cell wall synthesis of Arabidopsis and the findings were confirmed in planta by bimolecular fluorescence complementation (BiFC) assay. Results indicated that although all CESA proteins can interact with each other, only CESA4 is able to form homodimers. A model is proposed for the secondary rosette structure. The RING-motif proved not to be essential for the interaction between the CESA proteins

KW - arabidopsis

KW - proteins

KW - genes

KW - identification

KW - biosynthesis

KW - domains

KW - system

KW - plants

KW - cdna

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