Interaction between the moss Physcomitrella patens and Phytophthora

A novel pathosystem for live-cell imaging of subcellular defence

Elysa J.R. Overdijk, Jeroen De Keijzer, Deborah De Groot, Charikleia Schoina, Klaas Bouwmeester, Tijs Ketelaar, Francine Govers*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)

Abstract

Live-cell imaging of plant-pathogen interactions is often hampered by the tissue complexity and multicell layered nature of the host. Here, we established a novel pathosystem with the moss Physcomitrella patens as host for Phytophthora. The tip-growing protonema cells of this moss are ideal for visualizing interactions with the pathogen over time using high-resolution microscopy. We tested four Phytophthora species for their ability to infect P. patens and showed that P. sojae and P. palmivora were only rarely capable to infect P. patens. In contrast, P. infestans and P. capsici frequently and successfully penetrated moss protonemal cells, showed intracellular hyphal growth and formed sporangia. Next to these successful invasions, many penetration attempts failed. Here the pathogen was blocked by a barrier of cell wall material deposited in papilla-like structures, a defence response that is common in higher plants. Another common response is the upregulation of defence-related genes upon infection and also in moss we observed this upregulation in tissues infected with Phytophthora. For more advanced analyses of the novel pathosystem we developed a special set-up that allowed live-cell imaging of subcellular defence processes by high-resolution microscopy. With this set-up, we revealed that Phytophthora infection of moss induces repositioning of the nucleus, accumulation of cytoplasm and rearrangement of the actin cytoskeleton, but not of microtubules.

Original languageEnglish
Pages (from-to)171-180
JournalJournal of Microscopy
Volume263
Issue number2
DOIs
Publication statusPublished - 2016

Fingerprint

Bryopsida
Phytophthora
Bryophyta
Microscopy
Up-Regulation
Sporangia
Plant Cells
Infection
Actin Cytoskeleton
Microtubules
Cell Wall
Cytoplasm
Growth
Genes

Keywords

  • Live-cell imaging
  • Physcomitrella patens
  • Phytophthora capsici
  • Phytophthora infestans
  • Plant-pathogen interaction
  • Subcellular defence

Cite this

@article{da2e586bdcd84597a7649fbcb3a1e5c4,
title = "Interaction between the moss Physcomitrella patens and Phytophthora: A novel pathosystem for live-cell imaging of subcellular defence",
abstract = "Live-cell imaging of plant-pathogen interactions is often hampered by the tissue complexity and multicell layered nature of the host. Here, we established a novel pathosystem with the moss Physcomitrella patens as host for Phytophthora. The tip-growing protonema cells of this moss are ideal for visualizing interactions with the pathogen over time using high-resolution microscopy. We tested four Phytophthora species for their ability to infect P. patens and showed that P. sojae and P. palmivora were only rarely capable to infect P. patens. In contrast, P. infestans and P. capsici frequently and successfully penetrated moss protonemal cells, showed intracellular hyphal growth and formed sporangia. Next to these successful invasions, many penetration attempts failed. Here the pathogen was blocked by a barrier of cell wall material deposited in papilla-like structures, a defence response that is common in higher plants. Another common response is the upregulation of defence-related genes upon infection and also in moss we observed this upregulation in tissues infected with Phytophthora. For more advanced analyses of the novel pathosystem we developed a special set-up that allowed live-cell imaging of subcellular defence processes by high-resolution microscopy. With this set-up, we revealed that Phytophthora infection of moss induces repositioning of the nucleus, accumulation of cytoplasm and rearrangement of the actin cytoskeleton, but not of microtubules.",
keywords = "Live-cell imaging, Physcomitrella patens, Phytophthora capsici, Phytophthora infestans, Plant-pathogen interaction, Subcellular defence",
author = "Overdijk, {Elysa J.R.} and {De Keijzer}, Jeroen and {De Groot}, Deborah and Charikleia Schoina and Klaas Bouwmeester and Tijs Ketelaar and Francine Govers",
year = "2016",
doi = "10.1111/jmi.12395",
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volume = "263",
pages = "171--180",
journal = "Journal of Microscopy",
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Interaction between the moss Physcomitrella patens and Phytophthora : A novel pathosystem for live-cell imaging of subcellular defence. / Overdijk, Elysa J.R.; De Keijzer, Jeroen; De Groot, Deborah; Schoina, Charikleia; Bouwmeester, Klaas; Ketelaar, Tijs; Govers, Francine.

In: Journal of Microscopy, Vol. 263, No. 2, 2016, p. 171-180.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Interaction between the moss Physcomitrella patens and Phytophthora

T2 - A novel pathosystem for live-cell imaging of subcellular defence

AU - Overdijk, Elysa J.R.

AU - De Keijzer, Jeroen

AU - De Groot, Deborah

AU - Schoina, Charikleia

AU - Bouwmeester, Klaas

AU - Ketelaar, Tijs

AU - Govers, Francine

PY - 2016

Y1 - 2016

N2 - Live-cell imaging of plant-pathogen interactions is often hampered by the tissue complexity and multicell layered nature of the host. Here, we established a novel pathosystem with the moss Physcomitrella patens as host for Phytophthora. The tip-growing protonema cells of this moss are ideal for visualizing interactions with the pathogen over time using high-resolution microscopy. We tested four Phytophthora species for their ability to infect P. patens and showed that P. sojae and P. palmivora were only rarely capable to infect P. patens. In contrast, P. infestans and P. capsici frequently and successfully penetrated moss protonemal cells, showed intracellular hyphal growth and formed sporangia. Next to these successful invasions, many penetration attempts failed. Here the pathogen was blocked by a barrier of cell wall material deposited in papilla-like structures, a defence response that is common in higher plants. Another common response is the upregulation of defence-related genes upon infection and also in moss we observed this upregulation in tissues infected with Phytophthora. For more advanced analyses of the novel pathosystem we developed a special set-up that allowed live-cell imaging of subcellular defence processes by high-resolution microscopy. With this set-up, we revealed that Phytophthora infection of moss induces repositioning of the nucleus, accumulation of cytoplasm and rearrangement of the actin cytoskeleton, but not of microtubules.

AB - Live-cell imaging of plant-pathogen interactions is often hampered by the tissue complexity and multicell layered nature of the host. Here, we established a novel pathosystem with the moss Physcomitrella patens as host for Phytophthora. The tip-growing protonema cells of this moss are ideal for visualizing interactions with the pathogen over time using high-resolution microscopy. We tested four Phytophthora species for their ability to infect P. patens and showed that P. sojae and P. palmivora were only rarely capable to infect P. patens. In contrast, P. infestans and P. capsici frequently and successfully penetrated moss protonemal cells, showed intracellular hyphal growth and formed sporangia. Next to these successful invasions, many penetration attempts failed. Here the pathogen was blocked by a barrier of cell wall material deposited in papilla-like structures, a defence response that is common in higher plants. Another common response is the upregulation of defence-related genes upon infection and also in moss we observed this upregulation in tissues infected with Phytophthora. For more advanced analyses of the novel pathosystem we developed a special set-up that allowed live-cell imaging of subcellular defence processes by high-resolution microscopy. With this set-up, we revealed that Phytophthora infection of moss induces repositioning of the nucleus, accumulation of cytoplasm and rearrangement of the actin cytoskeleton, but not of microtubules.

KW - Live-cell imaging

KW - Physcomitrella patens

KW - Phytophthora capsici

KW - Phytophthora infestans

KW - Plant-pathogen interaction

KW - Subcellular defence

U2 - 10.1111/jmi.12395

DO - 10.1111/jmi.12395

M3 - Article

VL - 263

SP - 171

EP - 180

JO - Journal of Microscopy

JF - Journal of Microscopy

SN - 0022-2720

IS - 2

ER -