Interaction between root-knot nematodes and Solanum spp. : variation in pathogenicity, cytology, proteins and DNA = [De interactie tussen wortelknobbelnematoden en Solanum spp. : variatie in ziekteverwekkend vermogen, cytologie, eiwitten en DNA]

J.G. van der Beek

Research output: Thesisexternal PhD, WU

Abstract

<br/>This thesis describes genetic variation in the root-knot nematodes <em>Meloidogyne hapla, M. chitwoodi</em> and <em>M. fallax,</em> particularly with respect to their pathogenicity on <em>Solanum</em> spp. Significant differences in virulence and aggressiveness were shown to exist between and within these species. Evidence for the occurrence of pathotypes of <em>M. chitwoodi</em> on <em>S. bulbocastanum</em> was obtained. Differences in virulence corresponded to differences in overall genetic variation, revealed by 2-D protein electrophoresis. A distinct species classification for <em>Meloidogyne</em> spp. was obtained by AFLPs and 2-D electrophoresis. In mating experiments <em>M.</em><em>chitwoodi</em> and <em>M. fallax</em> appeared to be true biological species as testified by infertility of their hybrids. Abnormalities during meiosis in oocytes of an isolate of <em>M. hapla</em> and in spermatocytes of isolates of <em>M. fallax</em> resulted in limited sexual recombination. The combination of post-reductional meiosis and the fusion of the second polar body with the egg pronucleus is probably responsable for maintenance of heterozygosity in meiotic parthenogenetic <em>Meloidogyne.</em> The constant production of males in these populations makes the development of homogeneous isolates impossible. A method was described to conserve nematode germplasm by long-term preservation of juveniles in liquid nitrogen.
Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
Supervisors/Advisors
  • Hoekstra, R.F., Promotor, External person
  • van Silfhout, C.H., Promotor
  • Maas, P.W.T., Promotor, External person
Award date10 Oct 1997
Place of PublicationS.l.
Publisher
Print ISBNs9789054857280
Publication statusPublished - 1997

Keywords

  • root nodules
  • nodulation
  • plant parasitic nematodes
  • meloidogyne
  • plant pests
  • solanaceae
  • pathogenicity
  • genetic variation
  • cytology
  • proteins
  • dna
  • plant nematology
  • host parasite relationships
  • theses

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