Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus

Bertel Strandbygaard, Antonio Lavazza, Davide Lelli, Yannick Blanchard, Béatrice Grasland, Sophie Le Poder, Nicolas Rose, Falko Steinbach, Wim H.M. van der Poel, Frederik Widén, Graham J. Belsham, Anette Bøtner*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Porcine epidemic diarrhea virus (PEDV) has caused extensive economic losses to pig producers in many countries. It was recently introduced, for the first time, into North America and outbreaks have occurred again in multiple countries within Europe as well. To assess the properties of various diagnostic assays for the detection of PEDV infection, multiple panels of porcine sera have been shared and tested for the presence of antibodies against PEDV in an inter-laboratory ring trial. Different laboratories have used a variety of “in house” ELISAs and also one commercial assay. The sensitivity and specificity of each assay has been estimated using a Bayesian analysis applied to the ring trial results obtained with the different assays in the absence of a gold standard. Although different characteristics were found, it can be concluded that each of the assays used can detect infection of pigs at a herd level by either the early European strains of PEDV or the recently circulating strains (INDEL and non-INDEL). However, not all the assays seem suitable for demonstrating freedom from disease in a country. The results from individual animals, especially when the infection has occurred within an experimental situation, show more variation.

Original languageEnglish
Pages (from-to)151-160
JournalVeterinary Microbiology
Volume197
DOIs
Publication statusPublished - 2016

Fingerprint

Porcine epidemic diarrhea virus
antibodies
Swine
Antibodies
assays
Serum
swine
Bayes Theorem
Virus Diseases
North America
Infection
infection
Disease Outbreaks
Enzyme-Linked Immunosorbent Assay
Economics
Bayesian theory
Sensitivity and Specificity
gold
herds
enzyme-linked immunosorbent assay

Keywords

  • ELISA
  • PEDV
  • Porcine coronavirus
  • Ring trial
  • Serology

Cite this

Strandbygaard, B., Lavazza, A., Lelli, D., Blanchard, Y., Grasland, B., Poder, S. L., ... Bøtner, A. (2016). Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus. Veterinary Microbiology, 197, 151-160. https://doi.org/10.1016/j.vetmic.2016.11.020
Strandbygaard, Bertel ; Lavazza, Antonio ; Lelli, Davide ; Blanchard, Yannick ; Grasland, Béatrice ; Poder, Sophie Le ; Rose, Nicolas ; Steinbach, Falko ; van der Poel, Wim H.M. ; Widén, Frederik ; Belsham, Graham J. ; Bøtner, Anette. / Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus. In: Veterinary Microbiology. 2016 ; Vol. 197. pp. 151-160.
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title = "Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus",
abstract = "Porcine epidemic diarrhea virus (PEDV) has caused extensive economic losses to pig producers in many countries. It was recently introduced, for the first time, into North America and outbreaks have occurred again in multiple countries within Europe as well. To assess the properties of various diagnostic assays for the detection of PEDV infection, multiple panels of porcine sera have been shared and tested for the presence of antibodies against PEDV in an inter-laboratory ring trial. Different laboratories have used a variety of “in house” ELISAs and also one commercial assay. The sensitivity and specificity of each assay has been estimated using a Bayesian analysis applied to the ring trial results obtained with the different assays in the absence of a gold standard. Although different characteristics were found, it can be concluded that each of the assays used can detect infection of pigs at a herd level by either the early European strains of PEDV or the recently circulating strains (INDEL and non-INDEL). However, not all the assays seem suitable for demonstrating freedom from disease in a country. The results from individual animals, especially when the infection has occurred within an experimental situation, show more variation.",
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author = "Bertel Strandbygaard and Antonio Lavazza and Davide Lelli and Yannick Blanchard and B{\'e}atrice Grasland and Poder, {Sophie Le} and Nicolas Rose and Falko Steinbach and {van der Poel}, {Wim H.M.} and Frederik Wid{\'e}n and Belsham, {Graham J.} and Anette B{\o}tner",
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doi = "10.1016/j.vetmic.2016.11.020",
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Strandbygaard, B, Lavazza, A, Lelli, D, Blanchard, Y, Grasland, B, Poder, SL, Rose, N, Steinbach, F, van der Poel, WHM, Widén, F, Belsham, GJ & Bøtner, A 2016, 'Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus', Veterinary Microbiology, vol. 197, pp. 151-160. https://doi.org/10.1016/j.vetmic.2016.11.020

Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus. / Strandbygaard, Bertel; Lavazza, Antonio; Lelli, Davide; Blanchard, Yannick; Grasland, Béatrice; Poder, Sophie Le; Rose, Nicolas; Steinbach, Falko; van der Poel, Wim H.M.; Widén, Frederik; Belsham, Graham J.; Bøtner, Anette.

In: Veterinary Microbiology, Vol. 197, 2016, p. 151-160.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Inter-laboratory study to characterize the detection of serum antibodies against porcine epidemic diarrhoea virus

AU - Strandbygaard, Bertel

AU - Lavazza, Antonio

AU - Lelli, Davide

AU - Blanchard, Yannick

AU - Grasland, Béatrice

AU - Poder, Sophie Le

AU - Rose, Nicolas

AU - Steinbach, Falko

AU - van der Poel, Wim H.M.

AU - Widén, Frederik

AU - Belsham, Graham J.

AU - Bøtner, Anette

PY - 2016

Y1 - 2016

N2 - Porcine epidemic diarrhea virus (PEDV) has caused extensive economic losses to pig producers in many countries. It was recently introduced, for the first time, into North America and outbreaks have occurred again in multiple countries within Europe as well. To assess the properties of various diagnostic assays for the detection of PEDV infection, multiple panels of porcine sera have been shared and tested for the presence of antibodies against PEDV in an inter-laboratory ring trial. Different laboratories have used a variety of “in house” ELISAs and also one commercial assay. The sensitivity and specificity of each assay has been estimated using a Bayesian analysis applied to the ring trial results obtained with the different assays in the absence of a gold standard. Although different characteristics were found, it can be concluded that each of the assays used can detect infection of pigs at a herd level by either the early European strains of PEDV or the recently circulating strains (INDEL and non-INDEL). However, not all the assays seem suitable for demonstrating freedom from disease in a country. The results from individual animals, especially when the infection has occurred within an experimental situation, show more variation.

AB - Porcine epidemic diarrhea virus (PEDV) has caused extensive economic losses to pig producers in many countries. It was recently introduced, for the first time, into North America and outbreaks have occurred again in multiple countries within Europe as well. To assess the properties of various diagnostic assays for the detection of PEDV infection, multiple panels of porcine sera have been shared and tested for the presence of antibodies against PEDV in an inter-laboratory ring trial. Different laboratories have used a variety of “in house” ELISAs and also one commercial assay. The sensitivity and specificity of each assay has been estimated using a Bayesian analysis applied to the ring trial results obtained with the different assays in the absence of a gold standard. Although different characteristics were found, it can be concluded that each of the assays used can detect infection of pigs at a herd level by either the early European strains of PEDV or the recently circulating strains (INDEL and non-INDEL). However, not all the assays seem suitable for demonstrating freedom from disease in a country. The results from individual animals, especially when the infection has occurred within an experimental situation, show more variation.

KW - ELISA

KW - PEDV

KW - Porcine coronavirus

KW - Ring trial

KW - Serology

U2 - 10.1016/j.vetmic.2016.11.020

DO - 10.1016/j.vetmic.2016.11.020

M3 - Article

VL - 197

SP - 151

EP - 160

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

ER -