Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology

Clare F.J. Browning, Antonello Di Nardo, Lissie Henry, Tim Pollard, Lynne Hendry, Aurore Romey, Anthony Relmy, Phaedra Eble, Emiliana Brocchi, Santina Grazioli, Donald P. King, Anna B. Ludi*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0–95.2%) and 94.8% (95% CI: 93.1–96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

Original languageEnglish
Pages (from-to)933-937
Number of pages5
JournalJournal of Veterinary Diagnostic Investigation
Volume32
Issue number6
DOIs
Publication statusPublished - 1 Nov 2020

Keywords

  • ELISA
  • foot-and-mouth disease virus
  • inter-laboratory study
  • serology

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