Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology

Clare F.J. Browning; Antonello Di Nardo; Lissie Henry; Tim Pollard; Lynne Hendry; Aurore Romey; Anthony Relmy; Phaedra Eble; Emiliana Brocchi; Santina Grazioli; Donald P. King; Anna B. Ludi

doi:10.1177/1040638720962070

Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology

Clare F.J. Browning, Antonello Di Nardo, Lissie Henry, Tim Pollard, Lynne Hendry, Aurore Romey, Anthony Relmy, Phaedra Eble, Emiliana Brocchi, Santina Grazioli, Donald P. King, Anna B. Ludi^*

^*Corresponding author for this work

Virology & Molecular Biology

Research output: Contribution to journal › Article › Academic › peer-review

6 Citations (Scopus)

Abstract

Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0–95.2%) and 94.8% (95% CI: 93.1–96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

Original language	English
Pages (from-to)	933-937
Number of pages	5
Journal	Journal of Veterinary Diagnostic Investigation
Volume	32
Issue number	6
DOIs	https://doi.org/10.1177/1040638720962070
Publication status	Published - 1 Nov 2020

Keywords

ELISA
foot-and-mouth disease virus
inter-laboratory study
serology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://edepot.wur.nl/535065Licence: CC BY

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Browning, C. F. J., Di Nardo, A., Henry, L., Pollard, T., Hendry, L., Romey, A., Relmy, A., Eble, P., Brocchi, E., Grazioli, S., King, D. P., & Ludi, A. B. (2020). Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology. Journal of Veterinary Diagnostic Investigation, 32(6), 933-937. https://doi.org/10.1177/1040638720962070

@article{94a77447b5eb4aabb66b8ec1ed5eacc5,

title = "Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology",

abstract = "Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0–95.2%) and 94.8% (95% CI: 93.1–96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.",

keywords = "ELISA, foot-and-mouth disease virus, inter-laboratory study, serology",

author = "Browning, {Clare F.J.} and {Di Nardo}, Antonello and Lissie Henry and Tim Pollard and Lynne Hendry and Aurore Romey and Anthony Relmy and Phaedra Eble and Emiliana Brocchi and Santina Grazioli and King, {Donald P.} and Ludi, {Anna B.}",

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Browning, CFJ, Di Nardo, A, Henry, L, Pollard, T, Hendry, L, Romey, A, Relmy, A, Eble, P, Brocchi, E, Grazioli, S, King, DP & Ludi, AB 2020, 'Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology', Journal of Veterinary Diagnostic Investigation, vol. 32, no. 6, pp. 933-937. https://doi.org/10.1177/1040638720962070

TY - JOUR

T1 - Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology

AU - Browning, Clare F.J.

AU - Di Nardo, Antonello

AU - Henry, Lissie

AU - Pollard, Tim

AU - Hendry, Lynne

AU - Romey, Aurore

AU - Relmy, Anthony

AU - Eble, Phaedra

AU - Brocchi, Emiliana

AU - Grazioli, Santina

AU - King, Donald P.

AU - Ludi, Anna B.

PY - 2020/11/1

Y1 - 2020/11/1

N2 - Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0–95.2%) and 94.8% (95% CI: 93.1–96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

AB - Serologic assays used to detect antibodies to nonstructural proteins (NSPs) of foot-and-mouth disease virus (FMDV) are used for disease surveillance in endemic countries, and are essential to providing evidence for freedom of the disease with or without vaccination and to recover the free status of a country after an outbreak. In a 5-site inter-laboratory study, we compared the performance of 2 commercial NSP ELISA kits (ID Screen FMD NSP ELISA single day [short] and overnight protocols, ID.Vet; PrioCHECK FMDV NS antibody ELISA, Thermo Fisher Scientific). The overall concordance between the PrioCHECK and ID Screen test was 93.8% (95% CI: 92.0–95.2%) and 94.8% (95% CI: 93.1–96.1%) for the overnight and short ID Screen incubation protocols, respectively. Our results indicate that the assays (including the 2 different formats of the ID Screen test) can be used interchangeably in post-outbreak serosurveillance.

KW - ELISA

KW - foot-and-mouth disease virus

KW - inter-laboratory study

KW - serology

U2 - 10.1177/1040638720962070

DO - 10.1177/1040638720962070

M3 - Article

C2 - 33025858

AN - SCOPUS:85092163595

SN - 1040-6387

VL - 32

SP - 933

EP - 937

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

IS - 6

ER -

Inter-laboratory comparison of 2 ELISA kits used for foot-and-mouth disease virus nonstructural protein serology

Abstract

Keywords

UN SDGs

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