Inter- and intramolecular dynamics of pyrenyl lipids in bilayer membranes from time-resolved fluorescence spectroscopy

E.G. Novikov, A.J.W.G. Visser

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

The analysis of time-resolved fluorescence of monopyrenyl phospholipid monomer and excimer emission is reviewed using a model based on the diffusion equation for bimolecular reactions in two-dimensional planar membrane bilayers. This aspect is illustrated by the analysis of a real experiment. The method can also be extended to short-range diffusion, which can be measured for dipyrenyl phospholipids in membrane bilayers. The latter aspect is illustrated by the analysis of a simulated experiment to show the experimental requirements to recover the desired parameters.
Original languageEnglish
Pages (from-to)297-305
JournalJournal of Fluorescence
Volume11
Issue number4
DOIs
Publication statusPublished - 2001

Keywords

  • Liposomes
  • Membrane bilayer
  • Pyrene monomer and excimer
  • Time-resolved fluorescence
  • Translation diffusion
  • Vesicles

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