Integrative Proteomic Profiling Reveals PRC2-Dependent Epigenetic Crosstalk Maintains Ground-State Pluripotency

Guido van Mierlo, René A.M. Dirks, Laura De Clerck, Arie B. Brinkman, Michelle Huth, Susan L. Kloet, Nehmé Saksouk, Leonie I. Kroeze, Sander Willems, Matthias Farlik, Christoph Bock, Joop H. Jansen, Dieter Deforce, Michiel Vermeulen, Jérôme Déjardin, Maarten Dhaenens, Hendrik Marks*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

32 Citations (Scopus)

Abstract

Marks and colleagues use integrative mass spectrometry to profile post-translational histone modifications and the chromatin-associated proteome in ground-state pluripotency. This reveals H3K27me3 and PRC2 as widespread hallmarks on euchromatin and heterochromatin. They show that ubiquitous chromatin-associated PRC2 protects the epigenome from priming, in particular from gaining DNA methylation.

Original languageEnglish
Pages (from-to)123-137.e8
JournalCell Stem Cell
Volume24
Issue number1
DOIs
Publication statusPublished - 3 Jan 2019
Externally publishedYes

Keywords

  • chromatin profiling
  • embryonic stem cells
  • epigenetics
  • ground-state pluripotency
  • H3K27me3
  • histone modifications

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