Integrating of lignocellulose-based and starch-rich biomass-based hydrogen production was investigated by mixing wheat straw hydrolysate with a wheat grain hydrolysate for improved fermentation. Enzymatic pretreatment and hydrolysis of wheat grains led to a hydrolysate with a sugar concentration of 93.4 g/L, while dilute-acid pretreatment and enzymatic hydrolysis of wheat straw led to a hydrolysate with sugar concentration 23.0 g/L. Wheat grain hydrolysate was not suitable for hydrogen production by the extreme thermophilic bacterium Caldicellulosiruptor saccharolyticus at glucose concentrations of 10 g/L or higher, and wheat straw hydrolysate showed good fermentability at total sugar concentrations of up to 10 g/L. The mixed hydrolysates showed good fermentability at the highest tested sugar concentration of 20 g/L, with a hydrogen production of 82–97% of that of the control with pure sugars. Mixing wheat grain hydrolysate with wheat straw hydrolysate would be beneficial for fermentative hydrogen production in a biorefinery.
- thermophiles caldicellulosiruptor-saccharolyticus
- dilute-acid pretreatment
- extreme thermophiles
- bioethanol production
Panagiotopoulos, I. A., Bakker, R. R. C., de Vrije, G. J., Claassen, P. A. M., & Koukios, E. G. (2013). Integration of first and second generation biofuels: Fermentative hydrogen production from wheat grain and straw. Bioresource Technology, 128, 345-350. https://doi.org/10.1016/j.biortech.2012.09.083