Integration of chicken genomic resources to enable whole-genome sequencing

J.A. Aerts, R.P.M.A. Crooijmans, S.J.B. Cornelissen, K. Hemmatian, A. Veenendaal, A. Jaader, J.J. van der Poel, V. Fillon, I. Vignal, M.A.M. Groenen

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18 Citations (Scopus)

Abstract

Different genomic resources in chicken were integrated through the Wageningen chicken BAC library. First, a BAC anchor map was created by screening this library with two sets of markers: microsatellite markers from the consensus linkage map and markers created from BAC end sequencing in chromosome walking experiments. Second, HindIII digestion fingerprints were created for all BACs of the Wageningen chicken BAC library. Third, cytogenetic positions of BACs were assigned by FISH. These integrated resources will facilitate further chromosome-walking experiments and whole-genome sequencing
Original languageEnglish
Pages (from-to)297-303
JournalCytogenetic and Genome Research
Volume102
Issue number1-4
DOIs
Publication statusPublished - 2003

Keywords

  • comparative map
  • physical map
  • markers
  • bac

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    Aerts, J. A., Crooijmans, R. P. M. A., Cornelissen, S. J. B., Hemmatian, K., Veenendaal, A., Jaader, A., van der Poel, J. J., Fillon, V., Vignal, I., & Groenen, M. A. M. (2003). Integration of chicken genomic resources to enable whole-genome sequencing. Cytogenetic and Genome Research, 102(1-4), 297-303. https://doi.org/10.1159/000075766