Integration of a pAL2-1 homologous mitochondrial plasmid associated with life span extension in Podospora anserina

M.F.P.M. Maas, C.H. Sellem, V. Rincheval, R.F. Hoekstra, A.J.M. Debets, A. Sainsard-Chanet

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

We isolated and characterized a novel spontaneous longevity mutant of Podospora anserina strain Wa32 carrying one of the pAL2-1 homologous mitochondrial plasmids. This mutant is at least ten fold longer-lived than the wild type, and is hence a formal suppressor of both the regular and the `plasmid-based¿ senescence process. We show that the longevity trait is maternally inherited and coincides with the presence of a copy of the plasmid integrated in the 5¿ UTR of the mitochondrial Complex I genes nd2 and nd3. This mutation is associated with complex alterations in the respiratory chain, including a dispensable induction of the alternative oxidase. It is also associated with a stabilization of the mitochondrial chromosome and a reduction of the overall cellular level of reactive oxygen species.
Original languageEnglish
Pages (from-to)659-671
JournalFungal Genetics and Biology
Volume44
Issue number7
DOIs
Publication statusPublished - 2007

Keywords

  • cytochrome-c-oxidase
  • long-lived mutant
  • alternative oxidase
  • complex-i
  • neurospora-intermedia
  • dna rearrangements
  • senescence
  • sequence
  • amplification
  • respiration

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