Insulin modulates the secretion of proteins from mature 3T3-L1 adipocytes: a role for transcriptional regulation of processing

P. Wang, J. Keijer, J.E. Bunschoten, F. Bouwman, J. Renes, E. Mariman

    Research output: Contribution to journalArticleAcademicpeer-review

    37 Citations (Scopus)

    Abstract

    Aims/hypothesis Under conditions of insulin resistance and type 2 diabetes, fat cells are subjected to increased levels of insulin, which may have a major impact on the secretion of adipokines. Materials and methods Using transcriptomics and proteomics, we investigated how insulin affects the transcription and protein secretion profile of mature 3T3-L1 adipocytes. Results We found that insulin has a significant impact on protein secretion of 3T3-L1 adipocytes. However, transcription is not the major regulation point for these secreted proteins. For extracellular matrix components, our data suggest that the mRNA level of processing enzymes, but not of target proteins, is the regulating point at which insulin stimulates secretion and function of the relevant proteins. Among these enzymes, we report a novel finding, namely that sulfatase 2 gene is regulated by insulin, which may induce a functional change in cultured adipocytes. Conclusions/interpretation We propose that enhancement of protein processing and secretion rather than transcription of the secreted protein genes is part of the strategic role of insulin in the induction of cellular responses
    Original languageEnglish
    Pages (from-to)2453-2462
    JournalDiabetologia
    Volume49
    Issue number10
    DOIs
    Publication statusPublished - 2006

    Keywords

    • heparan-sulfate proteoglycans
    • extracellular-matrix
    • diabetes-mellitus
    • cells
    • metabolism
    • expression
    • resistance
    • molecules
    • apoptosis
    • pathways

    Fingerprint

    Dive into the research topics of 'Insulin modulates the secretion of proteins from mature 3T3-L1 adipocytes: a role for transcriptional regulation of processing'. Together they form a unique fingerprint.

    Cite this