Insulin acutely upregulates protein expression of the fatty acid transporter CD36 in human skeletal muscle in vivo

E. Corpeleijn*, M.M.A.L. Pelsers, S. Soenen, M. Mensink, F.G. Bouwman, M.E. Kooi, W.H.M. Saris, J.F.C. Glatz, E.E. Blaak

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

19 Citations (Scopus)

Abstract

Enhanced fatty acid uptake may lead to the accumulation of lipid intermediates. This is related to insulin resistance and type 2 diabetes mellitus. Rodent studies suggest that fatty acid transporters are acutely regulated by insulin. We investigated differences in fatty acid transporter content before and at the end of a hyperinsulinemic euglycemic clamp in skeletal muscle (m. vastus lateralis) of obese, glucose-intolerant men (IGT) and obese normal glucose tolerant controls (NGT). The fatty acid transporter FAT/CD36 protein content increased 1.5-fold (P < 0.05) after 3-hrs of insulin stimulation with no difference between IGT and control subjects. No change was seen in cytosolic fatty acid binding protein (FABPc) protein content. The increase in FAT/CD36 protein content was positively related to insulin resistance as measured during the clamp (r = 0.56, P < 0.05). An increase in FAT/CD36 protein content in skeletal muscle may result in a higher fractional extraction of fatty acids (larger relative uptake) after a meal, enhancing triglyceride accumulation in the muscle. We conclude that also in obese humans the FAT/CD36 protein content in skeletal muscle is dynamically regulated by insulin in vivo on the short term.

Original languageEnglish
Pages (from-to)77-83
Number of pages7
JournalJournal of Physiology and Pharmacology
Volume59
Issue number1
Publication statusPublished - 1 Mar 2008
Externally publishedYes

Keywords

  • FAT/CD36
  • Impaired glucose tolerance
  • Insulin action
  • Lipid metabolism
  • Obesity
  • Skeletal muscle

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    Corpeleijn, E., Pelsers, M. M. A. L., Soenen, S., Mensink, M., Bouwman, F. G., Kooi, M. E., Saris, W. H. M., Glatz, J. F. C., & Blaak, E. E. (2008). Insulin acutely upregulates protein expression of the fatty acid transporter CD36 in human skeletal muscle in vivo. Journal of Physiology and Pharmacology, 59(1), 77-83.