Innate immune training and metabolic reprogramming in primary monocytes of broiler and laying hens

Michel B. Verwoolde, Robin H.G.A. van den Biggelaar, Ger de Vries Reilingh, Joop A.J. Arts, Jürgen van Baal, Aart Lammers*, Christine A. Jansen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Recently, we have reported trained innate immunity in laying chicken monocytes. In the present study, we further investigated trained innate immunity of monocytes in layers and broilers. Monocytes of both breeds isolated from blood were trained in vitro with β-glucan, rec-chicken IL-4 or a combination of both, and restimulated with lipopolysaccharide (LPS), after which inflammation and metabolism-related responses were measured. Training of laying and broiler hen monocytes resulted in increased mRNA levels of IL-1β, iNOS and HIF-1α, but enhanced surface expression of CD40 and NO production was only observed in layers. Our in vitro study demonstrates that monocytes from different genetic backgrounds can be trained. However, the observed differences suggest a differential effect on immune functionality associated with innate training. Whether these differences in immune functions between layers and broilers have effect on disease resistance remains to be elucidated.

Original languageEnglish
Article number103811
JournalDevelopmental and Comparative Immunology
Volume114
Early online date1 Aug 2020
DOIs
Publication statusPublished - Jan 2021

Keywords

  • Chickens
  • Immune training
  • Lipopolysaccharide
  • Metabolism
  • Monocytes
  • β-glucan

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