Injectable nanoparticle-loaded hydrogen system for local delivery of sodium alendronate

U. Posadowska, M. Parizek, E. Filova, M.K. Wlodarczyk-Biegun, M.M.G. Kamperman, L. Bacakova, E. Pamula

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41 Citations (Scopus)


Systemic administration of bisphosphonates, e.g. sodium alendronate (Aln) is characterized by extremely low bioavailability and high toxicity. To omit aforementioned drawbacks an injectable system for the intra-bone delivery of Aln based on Aln-loaded nanoparticles (NPs-Aln) suspended in a hydrogel matrix (gellan gum, GG) was developed. Aln was encapsulated in poly(lactide-co-glycolide) (PLGA 85:15) by solid–oil–water emulsification. Drug release tests showed that within 25 days all the encapsulated drug was released from NPs-Aln and the release rate was highest at the beginning and decreased with time. In contrast, by suspending NPs-Aln in a GG matrix, the release rate was significantly lower and more constant in time. The GG–NPs-Aln system was engineered to be easily injectable and was able to reassemble its structure after extrusion as shown by rheological measurements. Invitro studies showed that the GG–NPs-Aln was cytocompatible with MG-63 osteoblast-like cells and it inhibited RANKL-mediated osteoclastic differentiation of RAW 264.7 cells. The injectability, the sustained local delivery of small doses of Aln and the biological activity render the GG–NPs-Aln system promising for the local treatment of osteoporosis and other bone tissue disorders.
Original languageEnglish
Pages (from-to)31-40
JournalInternational Journal of Pharmaceutics
Issue number1
Publication statusPublished - 2015


  • drug-delivery
  • osteoclast formation
  • controlled-release
  • bone
  • gellan
  • cytotoxicity
  • formulation
  • cells
  • microspheres
  • therapeutics


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