Inhibitory effect of rapamycin and dexamethasone on production of IL-17 and IFN-gamma in Vogt-Koyanagi-Harada patients

K. Yang, J. Wen, X. Liu, A. Kijlstra, L. Chen, W. Chi, H. Zhou, X. Huang, P. Yang

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

Aims: To evaluate the effect of rapamycin (RAPA) and dexamethasone (DEX) on the production of IL-17 and IFN-gamma by peripheral blood mononuclear cells (PBMCs) from Vogt-Koyanagi-Harada (VKH) patients and healthy individuals. Methods: Blood samples were drawn from 10 active VKH patients and 10 healthy individuals. PBMCs were cultured with or without anti-CD3 and anti-CD28 antibodies in the presence or absence of different concentrations of RAPA or DEX for 72 h. IL-17 and IFN-gamma concentrations in the supernatants were measured by enzyme-linked immunosorbent assay (ELISA). Results: The expression of IL-17 and IFN-gamma was significantly increased in active VKH patients compared with that in healthy controls. Both RAPA and DEX were able to significantly inhibit the production of IL-17 and IFN-gamma by PBMCs from patients and healthy controls. RAPA was able to completely inhibit IL-17 production at a dosage of 10 ng/ml but only partially suppressed IFN-gamma production even at a much higher concentration (1000 ng/ml). DEX inhibited the production of both IL-17 and IFN-gamma by approximately 70%. Conclusions: This study indicates that both RAPA and DEX inhibit the production of IL-17 and IFN-gamma by PBMCs. RAPA is much stronger in inhibiting the production of IL-17 than DEX.
Original languageEnglish
Pages (from-to)249-253
JournalBritish Journal of Ophthalmology
Volume93
Issue number2
DOIs
Publication statusPublished - 2009

Keywords

  • t-cell-activation
  • interferon-gamma
  • in-vitro
  • autoimmune uveoretinitis
  • immunosuppressive drugs
  • signal-transduction
  • cytokine production
  • modulation
  • expression
  • disease

Fingerprint Dive into the research topics of 'Inhibitory effect of rapamycin and dexamethasone on production of IL-17 and IFN-gamma in Vogt-Koyanagi-Harada patients'. Together they form a unique fingerprint.

Cite this