Inhibition of LPS-induced proinflammatory responses of J774.2 macrophages by immobilized enzymatically tailored pectins

M. Gallet, M. Vayssade, M. Morra, R.P. Verhoef, S. Perrone, G. Cascardo, P. Vigneron, H.A. Schols, M.D. Nagel

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Abstract

The surface of an implant device can be modified by immobilizing biological molecules on it to improve its integration into the host tissue. We have previously demonstrated that enzymatically tailored plant pectins are promising nanocoatings for biomaterials. This study investigates whether a coating of modified hairy region (rhamnogalacturonan-I) from apple pectin (MHR-a) which has anti-adhesive properties can inhibit the generation of inflammatory mediators by lipopolysaccharide (LPS)-activated macrophages. For that purpose, J774.2 murine macrophages were cultured for 24 h on MHR-a-coated Petri dishes and tissue culture polystyrene controls, with and without LPS. Cell morphology, cell growth, nitrite and TNF-a secretion were studied. The results indicate that MHR-a coating inhibits the LPS-induced activation of macrophages.
Original languageEnglish
Pages (from-to)2618-2622
JournalActa Biomaterialia
Volume5
DOIs
Publication statusPublished - 2009

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Keywords

  • hairy ramified regions
  • glinus-oppositifolius
  • rhamnogalacturonan-i
  • cell
  • polysaccharides
  • adhesion
  • activation
  • polymer
  • plant
  • vitro

Cite this

Gallet, M., Vayssade, M., Morra, M., Verhoef, R. P., Perrone, S., Cascardo, G., ... Nagel, M. D. (2009). Inhibition of LPS-induced proinflammatory responses of J774.2 macrophages by immobilized enzymatically tailored pectins. Acta Biomaterialia, 5, 2618-2622. https://doi.org/10.1016/j.actbio.2009.03.031