Inhibition of growth and metabolism of Chlorella and some other plant types by calcium dipicrylamine and other poisons

J.F. Bierhuizen

Research output: Thesisinternal PhD, WU

Abstract

If potassium is obtained from sea-water by precipitation with calcium dipicrylamine, potassium fertilizers and sea-water will be contaminated with a little dipicrylamine. The influence of calcium dipicrylamine on metabolism of Chlorella and some other aquatic and terrestrial plants was therefore studied. It inhibited photosynthesis even at lower concentrations (10 -6-10 -5M), than other inhibitors such as ethyl urethane, phenyl urethane, hydroxylamine, 2,4-dinitrophenol and sodium azide. Autotrophic growth was inhibited at almost the same concentration. But heterotrophic growth of Chlorella was less inhibited. Respiration was stimulated by up to 60 %, even at 10 -2M. At this concentration, glucose-stimulated respiration was 50 % inhibited but at least partly reversibly. The same degree of inhibition (not reversible by washing) was observed with light saturation as with light limitation in Chlorella photosynthesis. This suggested a specific enzyme- inhibitor, influencing certain dark reactions as well as the energy transfer in photosynthesis. Lower temperatures severely increased inhibition of photosynthesis in Chlorella.

Despite inhibition of growth and photosynthesis at low concentrations in water culture, the compound was less harmful in the field through the high adsorptive capacity of soil, especially of humus. Only with extreme artificial contamination was there a decrease in yield.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Wassink, E.C., Promotor
Award date5 Jul 1957
Place of PublicationWageningen
Publisher
Publication statusPublished - 1957
Externally publishedYes

Keywords

  • plant physiology
  • growth
  • plant development
  • metabolism
  • plant nutrition
  • assimilation
  • toxic substances
  • chemistry
  • indicator plants
  • chemical analysis
  • exposure
  • environmental degradation
  • kinetics
  • chemical factors
  • ecotoxicology

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