Inhibition of gap junctional intercellular communication between primary human smooth muscle cells by tumor necrosis factor

Tumor necrosis factor α (TNFα), a pleiotrophic cytokine present in atherosclerotic lesions, caused a dose-dependent and persistent reduction in gap junctional intercellular communication (GJIC) between primary human smooth muscle cells in vitro. A continuous presence of TNFα was required for this persistent inhibition. Pretreatment of smooth muscle cells with ascorbic acid, α-tocopherol or glutathione prevented this inhibition of GJIC by TNFα. The persistent blockage of GJIC by continuous exposure to TNFα suggests that TNFa may share some mechanistic similarities with exogenous tumor promoters. Furthermore, this reduction in GJIC by TNFa may provide an additional link between the processes of atherosclerosis and carcinogenesis. The protection afforded by antioxidant compounds suggests a role for active oxygen species in the promotion stage of atherosclerosis.