Inherent properties not conserved in other tenuiviruses increase priming and realignment cycles during transcription of Rice stripe virus

Xiaojuan Liu, Guihong Xiong, Ping Qiu, Zhenguo Du*, Richard Kormelink, Luping Zheng, Jie Zhang, Xinlun Ding, Liang Yang, Songbai Zhang, Zujian Wu

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

Two tenuiviruses Rice stripe virus (RSV) and Rice grassy stunt virus (RGSV) were found to co-infect rice with the same reovirus Rice ragged stunt virus (RRSV). During the co-infection, both tenuiviruses recruited 10-21 nucleotides sized capped-RNA leaders from the RRSV. A total of 245 and 102 RRSV-RGSV and RRSV-RSV chimeric mRNA clones, respectively, were sequenced. An analysis of the sequences suggested a scenario consistent with previously reported data on related viruses, in which capped leader RNAs having a 3' end complementary to the viral template are preferred and upon base pairing the leaders prime processive transcription directly or after one to several cycles of priming and realignment (repetitive prime-and-realign). Interestingly, RSV appeared to have a higher tendency to use repetitive prime-and-realign than RGSV even with the same leader derived from the same RRSV RNA. Combining with relevant data reported previously, this points towards an intrinsic feature of RSV.

Original languageEnglish
Pages (from-to)287-298
Number of pages12
JournalVirology
Volume496
DOIs
Publication statusPublished - 1 Sep 2016

Keywords

  • Cap-snatching
  • Co-infection
  • Plant-infecting reovirus
  • Prime-and-realign
  • Tenuivirus

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