Influence of the relative humidity on the morphology of inkjet printed spots of IgG on a non-porous substrate.

L.H. Mujawar, J.G.M. Kuerten, D.P. Siregar, A. van Amerongen, W. Norde

Research output: Contribution to journalArticleAcademicpeer-review

10 Citations (Scopus)


During the drying of inkjet printed droplets, the solute particles (IgG-Alexa-635 molecules) in the drop may distribute unevenly on the substrate, resulting in a “coffee-stain” spot morphology. In our study, we investigated the influence of the relative humidity on the distribution of inkjet printed fluorophore labeled IgG molecules on a polystyrene substrate. A theoretical model for an evaporating droplet was developed in order to predict the changes in the spot diameter, height and volume of a drying droplet. An experiment was performed where a sessile droplet was monitored using a CCD camera installed on a goniometer and good agreement was found between the experimental results and simulation data. We also compared the predicted morphology for an inkjet-printed microarray spot with the experimental results where IgG molecules were printed for various relative humidities. The spot morphology of the dried spots was analyzed by a confocal laser microscopy. At a lower relative humidity (i.e.,
Original languageEnglish
Pages (from-to)19380-19388
JournalRSC Advances : An international journal to further the chemical sciences
Issue number37
Publication statusPublished - 2014


  • experimental-verification
  • protein microarrays
  • dna microarrays
  • contact-angle
  • evaporation
  • surfaces
  • drops
  • performance
  • fabrication
  • adsorption


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