Influence of temperature fluctuations on Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in manure.

A.V. Semenov, A.H.C. van Bruggen, L.S. van Overbeek, A.J. Termorshuizen, A.M. Semenov

Research output: Contribution to journalArticleAcademicpeer-review

103 Citations (Scopus)

Abstract

The effects of four average temperatures (7, 16, 23 and 33°C) and daily oscillations with three amplitudes (0, ±4, ±7°C) on the survival of the enteropathogens Escherichia coli O157:H7 and Salmonella serovar Typhimurium were investigated in small microcosms. Manure was inoculated with a green fluorescent protein transformed strain of either pathogen at 107 cells g¿1 dry weight. Samples were collected immediately after inoculation, and 1 and 2 weeks after inoculation for E. coli O157:H7, and immediately and after 2 and 3 weeks for Salmonella serovar Typhimurium. Population densities were determined by dilution plating and direct counting. In addition, total bacterial CFUs were determined. Growth and survival data were fitted to a modified logistic model. Analysis of the estimated parameter values showed that E. coli O157:H7 survived for shorter periods of time and was more sensitive to competition by the native microbial community than Salmonella serovar Typhimurium. Survival of both pathogens significantly declined with increasing mean temperatures and with increasing amplitude in daily temperature oscillations. The results indicated that responses of enteropathogens to fluctuating temperatures cannot be deduced from temperature relationships determined under constant temperatures.
Original languageEnglish
Pages (from-to)419-428
JournalFEMS microbiology ecology
Volume60
Issue number3
DOIs
Publication statusPublished - 2007

Keywords

  • farmyard manure
  • escherichia coli
  • salmonella typhimurium
  • temperature
  • temperature resistance
  • pathogens
  • food safety
  • gastroenteritis
  • food poisoning
  • green fluorescent protein
  • aggregative behavior
  • nitrogen-content
  • bovine manure
  • united-states
  • amended soil
  • survival
  • growth
  • cattle

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