Influence of Protein Hydrolysis on the Growth Kinetics of ß-lg Fibrils

A. Kroes-Nijboer, P. Venema, J. Bouman, E. van der Linden

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49 Citations (Scopus)

Abstract

Recently it was found that protein hydrolysis is an important step in the formation of ß-lactoglobulin fibrils at pH 2 and elevated temperatures. The objective of the present study was to further investigate the influence of hydrolysis on the kinetics of fibril formation. Both the hydrolysis of ß-lactoglobulin and the growth of the fibrils were followed as a function of time and temperature, using SDS polyacrylamide gel electrophoresis and a Thioflavin T fluorescence assay. As an essential extension to existing models, the quantification of the effect of the hydrolysis on the fibrillar growth was established by a simple polymerization model including a hydrolysis step.
Original languageEnglish
Pages (from-to)5753-5761
JournalLangmuir
Volume27
Issue number10
DOIs
Publication statusPublished - 2011

Keywords

  • lactoglobulin gels
  • amyloid fibrils
  • ph 2
  • aggregation
  • heat
  • lysozyme
  • amyloidogenesis

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