TY - JOUR
T1 - Influence of nitrogen depletion in the growth of N. oleoabundans on the release of cellular components after beadmilling
AU - Günerken, Emre
AU - D'Hondt, Els
AU - Eppink, Michel
AU - Elst, Kathy
AU - Wijffels, Rene
PY - 2016
Y1 - 2016
N2 - Cell disruption by bead milling of Neochloris oleoabundans grown under nitrogen repleted (NR) and nitrogen depleted (ND) conditions was evaluated based on the release of biochemicals and biomass to the supernatant. Additionally, energy consumption for cell disruption was calculated per kg of released component. Bead milling of NR cells resulted on average in 34% (w/w) release of biochemicals into the supernatant, with a similar composition as the untreated microalgal biomass. With ND cells, the release was higher and more selective, i.e. 57%, 59%, 68% and 56% (w/w) of respectively biomass, proteins, carbohydrates and lipids whereof 92%, 57% and 46% (w/w) respectively of phospho-, glyco- and neutral lipids.
AB - Cell disruption by bead milling of Neochloris oleoabundans grown under nitrogen repleted (NR) and nitrogen depleted (ND) conditions was evaluated based on the release of biochemicals and biomass to the supernatant. Additionally, energy consumption for cell disruption was calculated per kg of released component. Bead milling of NR cells resulted on average in 34% (w/w) release of biochemicals into the supernatant, with a similar composition as the untreated microalgal biomass. With ND cells, the release was higher and more selective, i.e. 57%, 59%, 68% and 56% (w/w) of respectively biomass, proteins, carbohydrates and lipids whereof 92%, 57% and 46% (w/w) respectively of phospho-, glyco- and neutral lipids.
KW - Biochemical release
KW - Cell disruption
KW - Microalgae
KW - Nitrogen depletion
U2 - 10.1016/j.biortech.2016.04.072
DO - 10.1016/j.biortech.2016.04.072
M3 - Article
AN - SCOPUS:84964394208
SN - 0960-8524
VL - 214
SP - 89
EP - 95
JO - Bioresource Technology
JF - Bioresource Technology
ER -
