Influence of aerobic and anaerobic conditions on survival of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in Luria-Bertani broth, farm-yard manure and slurry

A.V. Semenov, L.S. van Overbeek, A.J. Termorshuizen, A.H.C. van Bruggen

Research output: Contribution to journalArticleAcademicpeer-review

51 Citations (Scopus)

Abstract

The influence of aerobic and anaerobic conditions on the survival of the enteropathogens Escherichia coli O157:H7 and Salmonella serovar Typhimurium was investigated in microcosms with broth, cattle manure or slurry. These substrates were inoculated with a green fluorescent protein transformed strain of the enteropathogens at 107 cells g-1 dry weight. Survival data was fitted to the Weibull model. The survival curves in aerobic conditions generally showed a concave curvature, while the curvature was convex in anaerobic conditions. The estimated survival times showed that E. coli O157:H7 survived significantly longer under anaerobic than under aerobic conditions. Survival ranged from approximately. 2 weeks for aerobic manure and slurry to more than six months for anaerobic manure at 16 °C. On average, in 56.3% of the samplings, the number of recovered E. coli O157:H7 cells by anaerobic incubation of Petri plates was significantly (p <0.05) higher in comparison with aerobic incubation. Survival of Salmonella serovar Typhimurium was not different between aerobic and anaerobic storage of LB broth or manure as well as between aerobic and anaerobic incubation of Petri dishes. The importance of changes in microbial community and chemical composition of manure and slurry was distinguished for the survival of E. coli O157:H7 in different oxygen conditions.
Original languageEnglish
Pages (from-to)780-787
JournalJournal of Environmental Management
Volume92
Issue number3
DOIs
Publication statusPublished - 2011

Keywords

  • gradient gel-electrophoresis
  • amended soil
  • cow manure
  • united-states
  • aggregative behavior
  • o157-h7 survival
  • fresh produce
  • ribosomal-rna
  • dairy manure
  • swine manure

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