Influence of acylation on the adsorption of GLP-2 to hydrophobic surfaces

C. Pinholt, S.J. Kapp, J.T. Bukrinsky, S. Hostrup, S. Frokjer, W. Norde, L. Jorgensen

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Acylation of proteins with a fatty acid chain has proven useful for prolonging the plasma half-lives of proteins. In formulation of acylated protein drugs, knowledge about the effect of acylation with fatty acids on the adsorption behaviour of proteins at interfaces will be valuable. The aim of this work was to study the effect of acylation on the adsorption of GLP-2 from aqueous solution to a hydrophobic surface by comparing the adsorption of the 3766 Da GLP-2 with that of a GLP-2 variant acylated with a 16-carbon fatty acid chain through a ß-alanine linker. Adsorption of GLP-2 and acylated GLP-2 were studied with isothermal titration calorimetry, fixed-angle optical reflectometry and total internal reflection fluorescence. Furthermore, the effect of acylation of GLP-2 on the secondary structure was studied with Far-UV CD. Acylation was observed to have several effects on the adsorption of GLP-2. Acylation increased the amount of GLP-2 adsorbing per unit surface area and decreased the initial adsorption rate of GLP-2. Finally, acylation increased the strength of the adsorption, as judged by the lower fraction desorbing upon rinsing with buffer.
Original languageEnglish
Pages (from-to)63-71
JournalInternational Journal of Pharmaceutics
Volume440
DOIs
Publication statusPublished - 2013

Keywords

  • glucagon-like peptide-2
  • protein adsorption
  • reversible lipidization
  • albumin-binding
  • solid-surfaces
  • human insulin
  • fatty-acids
  • in-vivo
  • analog
  • desmopressin

Fingerprint

Dive into the research topics of 'Influence of acylation on the adsorption of GLP-2 to hydrophobic surfaces'. Together they form a unique fingerprint.

Cite this