Inflammation in the middle ear of children with recurrent or chronic otitis media is associated with bacterial load

K. Stol, D.A. Diavatopoulos, K. Graamans, J.A. Engel, W.J.G. Melchers, H.F.J. Savelkoul, J.P. Hays, A. Warris, P.W.M. Hermans

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Background: Viral upper respiratory tract infections have been described as an important factor in the development of otitis media (OM), although it is unclear whether they facilitate bacterial OM or can directly cause OM. To clarify the role of viral infections in OM, we compared the relative contribution of viruses and bacteria with the induction of inflammatory cytokine responses in the middle ear of children suffering from OM. Methods: Children up to 5 years of age, with recurrent or chronic episodes of OM and scheduled for ventilation tube insertion were enrolled in a prospective study. Middle ear fluids (n = 116) were collected during surgery, and quantitative polymerase chain reaction was performed to detect bacterial and viral otopathogens, that is, Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis and 15 respiratory viruses. Finally, concentrations of the inflammatory mediators interleukin (IL)-1 beta, IL-6, IL-8, IL-10, IL-17a and tumor necrosis factor-a were determined. Results: Middle ear fluids were clustered into 4 groups, based on the detection of viruses (28%), bacteria (27%), both bacteria and viruses (27%) or no otopathogens (19%). Bacterial detection was associated with significantly elevated concentrations of cytokines compared with middle ear fluids without bacteria (P <0.001 for all cytokines tested) in a bacterial load-dependent and species-dependent manner. In contrast, the presence of viruses was not associated with changes in cytokine values, and no synergistic effect between viral-bacterial coinfections was observed. Conclusions: The presence of bacteria, but not viruses, is associated with an increased inflammatory response in the middle ear of children with recurrent or chronic OM.
Original languageEnglish
Pages (from-to)1128-1134
JournalThe Pediatric Infectious Disease Journal
Volume31
Issue number11
DOIs
Publication statusPublished - 2012

Keywords

  • respiratory-syncytial-virus
  • real-time pcr
  • streptococcus-pneumoniae
  • haemophilus-influenzae
  • moraxella-catarrhalis
  • immunoregulatory cytokines
  • effusion
  • rhinovirus
  • infections
  • expression

Fingerprint Dive into the research topics of 'Inflammation in the middle ear of children with recurrent or chronic otitis media is associated with bacterial load'. Together they form a unique fingerprint.

Cite this