Infection of Arabidopsis thaliana by Phytophthora parasitica and identification of variation in host specificity

Y. Wang, Y. Meng, M. Zhang, X. Tong, Q. Wang, Y. Sun, J. Quan, F. Govers, W. Shan

Research output: Contribution to journalArticleAcademicpeer-review

44 Citations (Scopus)

Abstract

Oomycete pathogens cause severe damage to a wide range of agriculturally important crops and natural ecosystems. They represent a unique group of plant pathogens that are evolutionarily distant from true fungi. In this study, we established a new plant–oomycete pathosystem in which the broad host range pathogen Phytophthora parasitica was demonstrated to be capable of interacting compatibly with the model plant Arabidopsis thaliana. Water-soaked lesions developed on leaves within 3 days and numerous sporangia formed within 5 days post-inoculation of P. parasitica zoospores. Cytological characterization showed that P. parasitica developed appressoria-like swellings and penetrated epidermal cells directly and preferably at the junction between anticlinal host cell walls. Multiple haustoria-like structures formed in both epidermal cells and mesophyll cells 1 day post-inoculation of zoospores. Pathogenicity assays of 25 A. thaliana ecotypes with six P. parasitica strains indicated the presence of a natural variation in host specificity between A. thaliana and P. parasitica. Most ecotypes were highly susceptible to P. parasitica strains Pp014, Pp016 and Pp025, but resistant to strains Pp008 and Pp009, with the frequent appearance of cell wall deposition and active defence response-based cell necrosis. Gene expression and comparative transcriptomic analysis further confirmed the compatible interaction by the identification of up-regulated genes in A. thaliana which were characteristic of biotic stress. The established A. thaliana–P. parasitica pathosystem expands the model systems investigating oomycete–plant interactions, and will facilitate a full understanding of Phytophthora biology and pathology.
Original languageEnglish
Pages (from-to)187-201
JournalMolecular Plant Pathology
Volume12
Issue number2
DOIs
Publication statusPublished - 2011

Keywords

  • expressed sequence tags
  • downy mildew resistance
  • gene-expression
  • salicylic-acid
  • fusarium-graminearum
  • confers resistance
  • defense responses
  • genome sequence
  • var.-nicotianae
  • cell

Fingerprint Dive into the research topics of 'Infection of Arabidopsis thaliana by Phytophthora parasitica and identification of variation in host specificity'. Together they form a unique fingerprint.

Cite this