Induction of trained innate immunity in human monocytes by bovine milk and milk-derived immunoglobulin G

Marloes van Splunter, Thijs L.J. van Osch, Sylvia Brugman, Huub F.J. Savelkoul, Leo A.B. Joosten, Mihai G. Netea, R.J.J. van Neerven

Research output: Contribution to journalArticleAcademicpeer-review

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Abstract

Innate immune memory, also termed “trained immunity” in vertebrates, has been recently described in a large variety of plants and animals. In most cases, trained innate immunity is induced by pathogens or pathogen-associated molecular patterns (PAMPs), and is associated with long-term epigenetic, metabolic, and functional reprogramming. Interestingly, recent findings indicate that food components can mimic PAMPs effects and induce trained immunity. The aim of this study was to investigate whether bovine milk or its components can induce trained immunity in human monocytes. To this aim, monocytes were exposed for 24 h to β-glucan, Toll-like receptor (TLR)-ligands, bovine milk, milk fractions, bovine lactoferrin (bLF), and bovine Immunoglobulin G (bIgG). After washing away the stimulus and a resting period of five days, the cells were re-stimulated with TLR ligands and Tumor necrosis factor (TNF-) and interleukin (IL)-6 production was measured. Training with β-glucan resulted in higher cytokine production after TLR1/2, TLR4, and TLR7/8 stimulation. When monocytes trained with raw milk were re-stimulated with TLR1/2 ligand Pam3CSK4, trained cells produced more IL-6 compared to non-trained cells. Training with bIgG resulted in higher cytokine production after TLR4 and TLR7/8 stimulation. These results show that bovine milk and bIgG can induce trained immunity in human monocytes. This confirms the hypothesis that diet components can influence the long-term responsiveness of the innate immune system.

Original languageEnglish
Article number1378
JournalNutrients
Volume10
Issue number10
DOIs
Publication statusPublished - 27 Sep 2018

Fingerprint

immunoglobulin G
Innate Immunity
monocytes
Monocytes
Milk
Immunoglobulin G
immunity
milk
cattle
glucans
Immunity
interleukin-6
pathogens
cytokines
Glucans
Toll-Like Receptors
Ligands
resting periods
lactoferrin
tumor necrosis factors

Keywords

  • Bovine IgG
  • Bovine lactoferrin
  • Dietary compounds
  • Innate immune memory
  • Monocytes
  • Raw bovine milk
  • Trained immunity

Cite this

van Splunter, M., van Osch, T. L. J., Brugman, S., Savelkoul, H. F. J., Joosten, L. A. B., Netea, M. G., & van Neerven, R. J. J. (2018). Induction of trained innate immunity in human monocytes by bovine milk and milk-derived immunoglobulin G. Nutrients, 10(10), [1378]. https://doi.org/10.3390/nu10101378
van Splunter, Marloes ; van Osch, Thijs L.J. ; Brugman, Sylvia ; Savelkoul, Huub F.J. ; Joosten, Leo A.B. ; Netea, Mihai G. ; van Neerven, R.J.J. / Induction of trained innate immunity in human monocytes by bovine milk and milk-derived immunoglobulin G. In: Nutrients. 2018 ; Vol. 10, No. 10.
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abstract = "Innate immune memory, also termed “trained immunity” in vertebrates, has been recently described in a large variety of plants and animals. In most cases, trained innate immunity is induced by pathogens or pathogen-associated molecular patterns (PAMPs), and is associated with long-term epigenetic, metabolic, and functional reprogramming. Interestingly, recent findings indicate that food components can mimic PAMPs effects and induce trained immunity. The aim of this study was to investigate whether bovine milk or its components can induce trained immunity in human monocytes. To this aim, monocytes were exposed for 24 h to β-glucan, Toll-like receptor (TLR)-ligands, bovine milk, milk fractions, bovine lactoferrin (bLF), and bovine Immunoglobulin G (bIgG). After washing away the stimulus and a resting period of five days, the cells were re-stimulated with TLR ligands and Tumor necrosis factor (TNF-) and interleukin (IL)-6 production was measured. Training with β-glucan resulted in higher cytokine production after TLR1/2, TLR4, and TLR7/8 stimulation. When monocytes trained with raw milk were re-stimulated with TLR1/2 ligand Pam3CSK4, trained cells produced more IL-6 compared to non-trained cells. Training with bIgG resulted in higher cytokine production after TLR4 and TLR7/8 stimulation. These results show that bovine milk and bIgG can induce trained immunity in human monocytes. This confirms the hypothesis that diet components can influence the long-term responsiveness of the innate immune system.",
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Induction of trained innate immunity in human monocytes by bovine milk and milk-derived immunoglobulin G. / van Splunter, Marloes; van Osch, Thijs L.J.; Brugman, Sylvia; Savelkoul, Huub F.J.; Joosten, Leo A.B.; Netea, Mihai G.; van Neerven, R.J.J.

In: Nutrients, Vol. 10, No. 10, 1378, 27.09.2018.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - van Splunter, Marloes

AU - van Osch, Thijs L.J.

AU - Brugman, Sylvia

AU - Savelkoul, Huub F.J.

AU - Joosten, Leo A.B.

AU - Netea, Mihai G.

AU - van Neerven, R.J.J.

PY - 2018/9/27

Y1 - 2018/9/27

N2 - Innate immune memory, also termed “trained immunity” in vertebrates, has been recently described in a large variety of plants and animals. In most cases, trained innate immunity is induced by pathogens or pathogen-associated molecular patterns (PAMPs), and is associated with long-term epigenetic, metabolic, and functional reprogramming. Interestingly, recent findings indicate that food components can mimic PAMPs effects and induce trained immunity. The aim of this study was to investigate whether bovine milk or its components can induce trained immunity in human monocytes. To this aim, monocytes were exposed for 24 h to β-glucan, Toll-like receptor (TLR)-ligands, bovine milk, milk fractions, bovine lactoferrin (bLF), and bovine Immunoglobulin G (bIgG). After washing away the stimulus and a resting period of five days, the cells were re-stimulated with TLR ligands and Tumor necrosis factor (TNF-) and interleukin (IL)-6 production was measured. Training with β-glucan resulted in higher cytokine production after TLR1/2, TLR4, and TLR7/8 stimulation. When monocytes trained with raw milk were re-stimulated with TLR1/2 ligand Pam3CSK4, trained cells produced more IL-6 compared to non-trained cells. Training with bIgG resulted in higher cytokine production after TLR4 and TLR7/8 stimulation. These results show that bovine milk and bIgG can induce trained immunity in human monocytes. This confirms the hypothesis that diet components can influence the long-term responsiveness of the innate immune system.

AB - Innate immune memory, also termed “trained immunity” in vertebrates, has been recently described in a large variety of plants and animals. In most cases, trained innate immunity is induced by pathogens or pathogen-associated molecular patterns (PAMPs), and is associated with long-term epigenetic, metabolic, and functional reprogramming. Interestingly, recent findings indicate that food components can mimic PAMPs effects and induce trained immunity. The aim of this study was to investigate whether bovine milk or its components can induce trained immunity in human monocytes. To this aim, monocytes were exposed for 24 h to β-glucan, Toll-like receptor (TLR)-ligands, bovine milk, milk fractions, bovine lactoferrin (bLF), and bovine Immunoglobulin G (bIgG). After washing away the stimulus and a resting period of five days, the cells were re-stimulated with TLR ligands and Tumor necrosis factor (TNF-) and interleukin (IL)-6 production was measured. Training with β-glucan resulted in higher cytokine production after TLR1/2, TLR4, and TLR7/8 stimulation. When monocytes trained with raw milk were re-stimulated with TLR1/2 ligand Pam3CSK4, trained cells produced more IL-6 compared to non-trained cells. Training with bIgG resulted in higher cytokine production after TLR4 and TLR7/8 stimulation. These results show that bovine milk and bIgG can induce trained immunity in human monocytes. This confirms the hypothesis that diet components can influence the long-term responsiveness of the innate immune system.

KW - Bovine IgG

KW - Bovine lactoferrin

KW - Dietary compounds

KW - Innate immune memory

KW - Monocytes

KW - Raw bovine milk

KW - Trained immunity

U2 - 10.3390/nu10101378

DO - 10.3390/nu10101378

M3 - Article

VL - 10

JO - Nutrients

JF - Nutrients

SN - 2072-6643

IS - 10

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ER -