Induction of the cholesterol biosynthesis pathway in differentiated Caco-2 cells bij the potato glycoalkaoid a-chaconine

T. Mandiminka, H. Baykus, J.H. Poortman, C. Garza, H.A. Kuiper, A.A.C.M. Peijnenburg

    Research output: Contribution to journalArticleAcademicpeer-review

    15 Citations (Scopus)

    Abstract

    Glycoalkaloids are naturally occurring toxins in potatoes, which at high levels may induce toxic effects in humans, mainly on the gastrointestinal tract by cell membrane disruption. In order to better understand the molecular mechanisms underlying glycoalkaloid toxicity, we examined the effects of ¿-chaconine on gene expression in the Caco-2 intestinal epithelial cell line using DNA microarrays. Caco-2 cells were exposed for 6 h to 10 ¿M ¿-chaconine in three independent experiments (randomized block design). The most prominent finding from our gene expression and pathway analyses was the upregulation of expression of several genes involved in cholesterol biosynthesis. This to some extent is in line with the literature-described mechanism of cell membrane disruption by glycoalkaloids. In addition, various growth factor signaling pathways were found to be significantly upregulated. This study is useful in understanding the mechanism(s) of ¿-chaconine toxicity, which may be extended to other potato glycoalkaloids more generally.
    Original languageEnglish
    Pages (from-to)1918-1927
    Number of pages10
    JournalFood and Chemical Toxicology
    Volume45
    Issue number10
    DOIs
    Publication statusPublished - 2007

    Keywords

    • induced membrane disruption
    • element-binding protein
    • growth-factor receptor
    • inhibitory factor mif
    • lipid rafts
    • steroidal glycoalkaloids
    • synergistic interaction
    • signal-transduction
    • solanum-tuberosum
    • activation

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