Induction of a tyrosinase mRNA in Agaricus bisporus upon treatment with a tolaasin preparation from Pseudomonas tolaasii

C. Soler Rivas, A.C. Moeller, N. Arpin, J. Olivier, H.J. Wichers

    Research output: Contribution to journalArticleAcademicpeer-review

    22 Citations (Scopus)

    Abstract

    In Agaricus bisporus infected with Pseudomonas tolaasii, or treated with a partially purified tolaasin extract or with purified tolaasin, induction of a specific mRNA of the two sequences encoding tyrosinase was found. Tyrosinase isoforms in samples treated with several concentrations of a partially purified tolaasin (PT) extract were visualized after Western analysis and staining with polyclonal tyrosinase-specific antibodies. Similar concentration of a 67 kDa latent tyrosinase was shown in all the samples including the water-treated control but the treatment with the PT extract caused the post-translational modification of the protein into an active tyrosinase of 43 kDa.
    Original languageEnglish
    Pages (from-to)95-99
    JournalPhysiological and Molecular Plant Pathology
    Volume58
    Issue number2
    DOIs
    Publication statusPublished - 2001

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