In Agaricus bisporus infected with Pseudomonas tolaasii, or treated with a partially purified tolaasin extract or with purified tolaasin, induction of a specific mRNA of the two sequences encoding tyrosinase was found. Tyrosinase isoforms in samples treated with several concentrations of a partially purified tolaasin (PT) extract were visualized after Western analysis and staining with polyclonal tyrosinase-specific antibodies. Similar concentration of a 67 kDa latent tyrosinase was shown in all the samples including the water-treated control but the treatment with the PT extract caused the post-translational modification of the protein into an active tyrosinase of 43 kDa.
Soler Rivas, C., Moeller, A. C., Arpin, N., Olivier, J., & Wichers, H. J. (2001). Induction of a tyrosinase mRNA in Agaricus bisporus upon treatment with a tolaasin preparation from Pseudomonas tolaasii. Physiological and Molecular Plant Pathology, 58(2), 95-99. https://doi.org/10.1006/pmpp.2000.0312