Abstract
In Agaricus bisporus infected with Pseudomonas tolaasii, or treated with a partially purified tolaasin extract or with purified tolaasin, induction of a specific mRNA of the two sequences encoding tyrosinase was found. Tyrosinase isoforms in samples treated with several concentrations of a partially purified tolaasin (PT) extract were visualized after Western analysis and staining with polyclonal tyrosinase-specific antibodies. Similar concentration of a 67 kDa latent tyrosinase was shown in all the samples including the water-treated control but the treatment with the PT extract caused the post-translational modification of the protein into an active tyrosinase of 43 kDa.
Original language | English |
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Pages (from-to) | 95-99 |
Journal | Physiological and Molecular Plant Pathology |
Volume | 58 |
Issue number | 2 |
DOIs | |
Publication status | Published - 2001 |
Keywords
- Agaricus bisporus
- mRNA induction
- Pseudomonas tolaasii
- Tolaasin
- Tyrosinase