Induction of a leaf specific geranylgeranyl pyrophosphate synthase and emission of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in tomato are dependent on both jasmonic acid and salicylic acid signaling pathways

K. Ament, C.C. Schie, H.J. Bouwmeester, M.A. Haring, R.C. Schuurink

Research output: Contribution to journalArticleAcademicpeer-review

69 Citations (Scopus)

Abstract

Two cDNAs encoding geranylgeranyl pyrophosphate (GGPP) synthases from tomato (Lycopersicon esculentum) have been cloned and functionally expressed in Escherichia coli. LeGGPS1 was predominantly expressed in leaf tissue and LeGGPS2 in ripening fruit and flower tissue. LeGGPS1 expression was induced in leaves by spider mite (Tetranychus urticae)-feeding and mechanical wounding in wild type tomato but not in the jasmonic acid (JA)-response mutant def-1 and the salicylic acid (SA)-deficient transgenic NahG line. Furthermore, LeGGPS1 expression could be induced in leaves of wild type tomato plants by JA- or methyl salicylate (MeSA)-treatment. In contrast, expression of LeGGPS2 was not induced in leaves by spider mite-feeding, wounding, JA- or MeSA-treatment. We show that emission of the GGPP-derived volatile terpenoid (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT) correlates with expression of LeGGPS1. An exception was MeSA-treatment, which resulted in induction of LeGGPS1 but not in emission of TMTT. We show that there is an additional layer of regulation, because geranyllinalool synthase, catalyzing the first dedicated step in TMTT biosynthesis, was induced by JA but not by MeSA.
Original languageEnglish
Pages (from-to)1197-1208
JournalPlanta
Volume224
Issue number5
DOIs
Publication statusPublished - 2006

Keywords

  • farnesyl diphosphate synthase
  • coenzyme-a reductase
  • arabidopsis-thaliana
  • carotenoid biosynthesis
  • differential induction
  • functional expression
  • molecular-cloning
  • methyl salicylate
  • chain elongation
  • wound response

Fingerprint Dive into the research topics of 'Induction of a leaf specific geranylgeranyl pyrophosphate synthase and emission of (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene in tomato are dependent on both jasmonic acid and salicylic acid signaling pathways'. Together they form a unique fingerprint.

Cite this